Cores Ziskoven, Pablo; Nogueira, Andressa V B; Gutierrez, Lorena S; Weusmann, Jens; Eick, Sigrun; Buduneli, Nurcan; Deschner, James (2023). Apelin Enhances the Effects of Fusobacterium nucleatum on Periodontal Ligament Cells In Vitro. International journal of molecular sciences, 24(5) MDPI 10.3390/ijms24054733
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This study aimed to explore effects of Fusobacterium nucleatum with or without apelin on periodontal ligament (PDL) cells to better understand pathomechanistic links between periodontitis and obesity. First, the actions of F. nucleatum on COX2, CCL2, and MMP1 expressions were assessed. Subsequently, PDL cells were incubated with F. nucleatum in the presence and absence of apelin to study the modulatory effects of this adipokine on molecules related to inflammation and hard and soft tissue turnover. Regulation of apelin and its receptor (APJ) by F. nucleatum was also studied. F. nucleatum resulted in elevated COX2, CCL2, and MMP1 expressions in a dose- and time-dependent manner. Combination of F. nucleatum and apelin led to the highest (p < 0.05) expression levels of COX2, CCL2, CXCL8, TNF-α, and MMP1 at 48 h. The effects of F. nucleatum and/or apelin on CCL2 and MMP1 were MEK1/2- and partially NF-κB-dependent. The combined effects of F. nucleatum and apelin on CCL2 and MMP1 were also observed at protein level. Moreover, F. nucleatum downregulated (p < 0.05) the apelin and APJ expressions. In conclusion, obesity could contribute to periodontitis through apelin. The local production of apelin/APJ in PDL cells also suggests a role of these molecules in the pathogenesis of periodontitis.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
04 Faculty of Medicine > School of Dental Medicine > Department of Periodontology 04 Faculty of Medicine > School of Dental Medicine > Periodontics Research |
UniBE Contributor: |
Eick, Sigrun |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
1422-0067 |
Publisher: |
MDPI |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
13 Mar 2023 12:38 |
Last Modified: |
14 Mar 2023 02:16 |
Publisher DOI: |
10.3390/ijms24054733 |
PubMed ID: |
36902162 |
Uncontrolled Keywords: |
APJ Fusobacterium nucleatum apelin obesity periodontal ligament cells periodontitis |
BORIS DOI: |
10.48350/179913 |
URI: |
https://boris.unibe.ch/id/eprint/179913 |
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