Yu, Shimin; Bd, Yinping Tian; Bd, Yan Wei; Bd, Mengge Feng; BMed, Sensen Li; BMed, Guoyong Tong; BMed, Zhouqing Yu; Miron, Richard J; Zhang, Yufeng; Yang, Zaibo; Wang, Yulan (2023). Early tissue and healing responses after maxillary sinus augmentation using horizontal platelet rich fibrin bone blocks. BMC Oral Health, 23(1), p. 589. BioMed Central 10.1186/s12903-023-03228-z
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BACKGROUND
The effects of horizontal platelet-rich fibrin (H-PRF) bone block on the healing and immune response during sinus augmentation have not been fully investigated histologically at early time points.
METHODS
Eighteenth male New Zealand white rabbits underwent bilateral sinus augmentation and were divided into two groups: deproteinized bovine bone mineral (DBBM) alone and H-PRF + DBBM (H-PRF bone block) group. Maxilla samples were collected at 3, 7 and 14 days post sinus augmentation procedures and analyzed using histological staining for the number of inflammatory cells, new blood vessels and evidence for early osteoclast bone turnover/remodeling. Furthermore, the effects of H-PRF bone blocks on the migration of osteoblasts and THP-1 macrophages were evaluated using a Transwell assay in vitro.
RESULTS
A higher number of immune cells were found in the H-PRF bone block group at 3 and 7 days post-surgery when compared to the DBBM alone group,most notably in the regions close to the mucosal lining and bone plates. Furthermore, a significantly greater number of new blood vessel formations and early signs of osteoclast development were found in the H-PRF bone block group at 14 days. The in vitro transwell assay further confirmed that culture medium from H-PRF bone block markedly promote the migration of osteoblasts and THP-1 macrophages.
CONCLUSIONS
The findings from this study have shown that H-PRF bone block is capable of increasing early immune cell infiltration leading to the acceleration of neovascularization and speeding the process of bone metabolism in vivo following maxillary sinus grafting with DBBM.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > School of Dental Medicine > Department of Periodontology |
UniBE Contributor: |
Miron, Richard John |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
1472-6831 |
Publisher: |
BioMed Central |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
25 Aug 2023 15:31 |
Last Modified: |
25 Aug 2023 15:41 |
Publisher DOI: |
10.1186/s12903-023-03228-z |
PubMed ID: |
37620826 |
Uncontrolled Keywords: |
Horizontal platelet-rich fibrin Inflammatory cells Maxillary sinus augmentation Vascularization |
BORIS DOI: |
10.48350/185729 |
URI: |
https://boris.unibe.ch/id/eprint/185729 |
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