Graichen, D A S; Gottstein, B; Matsumoto, J; Müller, N; Zanotto, P M A; Ayala, F J; Haag, K L (2007). Expression and diversity of Echinococcus multilocularis AgB genes in secondarily infected mice: evaluating the influence of T-cell immune selection on antigenic variation. Gene, 392(1-2), pp. 98-105. Amsterdam: Elsevier 10.1016/j.gene.2006.11.015
Full text not available from this repository. (Request a copy)The T-cell-mediated immune response exhibits a crucial function in the control of the intrahepatic proliferation of Echinococcus multilocularis larvae in mice and humans, both being natural intermediate hosts of the parasite. Antigen B (AgB), a metabolized Echinococcus spp. lipoprotein, contributes to the modulation of the T-cell immune response, and distinct sites of the corresponding AgB1, AgB3 and AgB4 genes were shown to be under positive selection pressure. Since several AgB gene variants are present in a single Echinococcus metacestode, we used secondary E. multilocularis infections in BALB/c and in athymic nude mice (devoid of T-cell responses) to analyze the effect of the cellular immune response on the expression and diversity of EmAgB1-EmAgB4 genes. We demonstrated hereby that EmAgB transcripts were less abundant in nude mice during the early phase of infection (at one month post-infection), and that EmAgB2 is simultaneously down-regulated when compared to the other three genes. A negative relationship exists between the level of transcription and diversity of EmAgB genes. Moreover, no excess of non-synonymous substitutions was found among the distinct EmAgB alleles from a single host. Together, these results pointed to the effect of purifying selection, which seemed to eliminate the detrimental AgB variants generated during the development of the metacestode within the peritoneal cavity of its intermediate host.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Parasitology |
UniBE Contributor: |
Gottstein, Bruno, Matsumoto, Jun, Müller, Norbert |
Subjects: |
600 Technology > 630 Agriculture |
ISSN: |
0378-1119 |
Publisher: |
Elsevier |
Language: |
English |
Submitter: |
Factscience Import |
Date Deposited: |
04 Oct 2013 14:45 |
Last Modified: |
05 Dec 2022 14:14 |
Publisher DOI: |
10.1016/j.gene.2006.11.015 |
PubMed ID: |
17208391 |
Web of Science ID: |
000246078300011 |
URI: |
https://boris.unibe.ch/id/eprint/18664 (FactScience: 871) |
Actions (login required)
 |
Edit item |