Exovesicles from human activated dendritic cells fuse with resting dendritic cells, allowing them to present alloantigens

Obregon, Carolina; Rothen-Rutishauser, Barbara; Gitahi, Stephen Kiama; Gehr, Peter; Nicod, Laurent P (2006). Exovesicles from human activated dendritic cells fuse with resting dendritic cells, allowing them to present alloantigens. American journal of pathology, 169(6), pp. 2127-36. New York, N.Y.: Elsevier 10.2323/alpath.2006.060453

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Dendritic cells (DCs) can release microvesicles, but the latter's numbers, size, and fate are unclear. Fluorescently labeled DCs were visualized by laser-scanning microscopy. Using a Surpass algorithm, we were able to identify and quantify per cell several hundred microvesicles released from the surface of stimulated DCs. We show that most of these microvesicles are not of endocytic origin but result from budding of the plasma membrane, hence their name, exovesicle. Using a double vital staining, we show that exovesicles isolated from activated DCs can fuse with the membrane of resting DCs, thereby allowing them to present alloantigens to lymphocytes. We concluded that, within a few hours from their release, exovesicles may amplify local or distant adaptive immunological response.

Item Type:	Journal Article (Original Article)
Division/Institute:	04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy 04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Pneumology
UniBE Contributor:	Gehr, Peter, Nicod, Laurent
ISSN:	0002-9440
ISBN:	17148675
Publisher:	Elsevier
Language:	English
Submitter:	Factscience Import
Date Deposited:	04 Oct 2013 14:46
Last Modified:	05 Dec 2022 14:14
Publisher DOI:	10.2323/alpath.2006.060453
PubMed ID:	17148675
Web of Science ID:	000242319700021
URI:	https://boris.unibe.ch/id/eprint/18944 (FactScience: 1216)