Jeran, Marko; Romolo, Anna; Spasovski, Vesna; Hočevar, Matej; Novak, Urban; Štukelj, Roman; Šuštar, Vid; Kisovec, Matic; Bedina Zavec, Apolonija; Kogej, Ksenija; Iglič, Aleš; Trebše, Polonca; Kralj-Iglič, Veronika (2023). Small Cellular Particles from European Spruce Needle Homogenate. International journal of molecular sciences, 24(5) MDPI 10.3390/ijms24054349
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Small cellular particles (SCPs) are being considered for their role in cell-to-cell communication. We harvested and characterized SCPs from spruce needle homogenate. SCPs were isolated by differential ultracentrifugation. They were imaged by scanning electron microscope (SEM) and cryogenic transmission electron microscope (cryo TEM), assessed for their number density and hydrodynamic diameter by interferometric light microscopy (ILM) and flow cytometry (FCM), total phenolic content (TPC) by UV-vis spectroscopy, and terpene content by gas chromatography-mass spectrometry (GC-MS). The supernatant after ultracentrifugation at 50,000× g contained bilayer-enclosed vesicles whereas in the isolate we observed small particles of other types and only a few vesicles. The number density of cell-sized particles (CSPs) (larger than 2 μm) and meso-sized particles (MSPs) (cca 400 nm-2 µm) was about four orders of magnitude lower than the number density of SCPs (sized below 500 nm). The average hydrodynamic diameter of SCPs measured in 10,029 SCPs was 161 ± 133 nm. TCP decreased considerably due to 5-day aging. Volatile terpenoid content was found in the pellet after 300× g. The above results indicate that spruce needle homogenate is a source of vesicles to be explored for potential delivery use.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
04 Faculty of Medicine > Department of Haematology, Oncology, Infectious Diseases, Laboratory Medicine and Hospital Pharmacy (DOLS) > Clinic of Medical Oncology |
UniBE Contributor: |
Novak, Urban |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
1422-0067 |
Publisher: |
MDPI |
Language: |
English |
Submitter: |
Teuta Bytyqi |
Date Deposited: |
12 Dec 2023 13:52 |
Last Modified: |
12 Dec 2023 13:52 |
Publisher DOI: |
10.3390/ijms24054349 |
PubMed ID: |
36901780 |
Uncontrolled Keywords: |
Picea abies cell-to-cell communication drug delivery exosomes extracellular particles extracellular vesicles interferometric light microscopy small cellular particles spruce needles |
BORIS DOI: |
10.48350/190068 |
URI: |
https://boris.unibe.ch/id/eprint/190068 |
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