da Costa, Natacha Malu Miranda; Parisi, Ludovica; Ghezzi, Benedetta; Elviri, Lisa; de Souza, Sergio Luis Scombatti; Novaes, Arthur Belém; de Oliveira, Paulo Tambasco; Macaluso, Guido Maria; Palioto, Daniela Bazan (2023). Anti-Fibronectin Aptamer Modifies Blood Clot Pattern and Stimulates Osteogenesis: An Ex Vivo Study. Biomimetics, 8(8) MDPI 10.3390/biomimetics8080582
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BACKGROUND
Scaffold (SCA) functionalization with aptamers (APT) provides adsorption of specific bioactive molecules on biomaterial surfaces. The aim of this study was to observe if SCA enriched with anti-fibronectin APT can favor coagulum (PhC) and osteoblasts (OSB) differentiation.
METHODS
20 μg of APT was functionalized on SCA by simple adsorption. For PhC formation, SCAs were inserted into rat calvaria defects for 17 h. Following proper transportation (buffer solution PB), OSBs (UMR-106 lineage) were seeded over PhC + SCAs with and without APT. Cells and PhC morphology, PhC cell population, protein labeling and gene expression were observed in different time points.
RESULTS
The APT induced higher alkaline phosphatase and bone sialoprotein immunolabeling in OSB. Mesenchymal stem cells, leukocytes and lymphocytes cells were detected more in the APT group than when scaffolds were not functionalized. Additionally, an enriched and dense fibrin network and different cell types were observed, with more OSB and white blood cells in PhC formed on SCA with APT. The gene expression showed higher transforming growth factor beta 1 (TGF-b1) detection in SCA with APT.
CONCLUSIONS
The SCA functionalization with fibronectin aptamers may alter key morphological and functional features of blood clot formation, and provides a selective expression of proteins related to osteo differentiation. Additionally, aptamers increase TGF-b1 gene expression, which is highly associated with improvements in regenerative therapies.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
04 Faculty of Medicine > School of Dental Medicine > Department of Orthodontics |
UniBE Contributor: |
Parisi, Ludovica |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
2313-7673 |
Publisher: |
MDPI |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
27 Dec 2023 16:21 |
Last Modified: |
27 Dec 2023 22:42 |
Publisher DOI: |
10.3390/biomimetics8080582 |
PubMed ID: |
38132522 |
Uncontrolled Keywords: |
aptamer blood clot hydrogel osteogenesis scaffold three dimensional |
BORIS DOI: |
10.48350/190729 |
URI: |
https://boris.unibe.ch/id/eprint/190729 |
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