Brunßen, Dominique; Suter, Beat (2024). Effects of unstable β-PheRS on food avoidance, growth, and development are suppressed by the appetite hormone CCHa2. Fly, 18(1) Taylor & Francis 10.1080/19336934.2024.2308737
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Effects_of_unstable_-PheRS_on_food_avoidance_growth_and_development_are_suppressed_by_the_appetite_hormone_CCHa2.pdf - Published Version Available under License Creative Commons: Attribution (CC-BY). Download (7MB) | Preview |
Amino acyl-tRNA synthetases perform diverse non-canonical functions aside from their essential role in charging tRNAs with their cognate amino acid. The phenylalanyl-tRNA synthetase (PheRS/FARS) is an α2β2 tetramer that is needed for charging the tRNAPhe for its translation activity. Fragments of the α-subunit have been shown to display an additional, translation-independent, function that activates growth and proliferation and counteracts Notch signalling. Here we show in Drosophila that overexpressing the β-subunit in the context of the complete PheRS leads to larval roaming, food avoidance, slow growth, and a developmental delay that can last several days and even prevents pupation. These behavioural and developmental phenotypes are induced by PheRS expression in CCHa2+ and Pros+ cells. Simultaneous expression of β-PheRS, α-PheRS, and the appetite-inducing CCHa2 peptide rescued these phenotypes, linking this β-PheRS activity to the appetite-controlling pathway. The fragmentation dynamic of the excessive β-PheRS points to β-PheRS fragments as possible candidate inducers of these phenotypes. Because fragmentation of human FARS has also been observed in human cells and mutations in human β-PheRS (FARSB) can lead to problems in gaining weight, Drosophila β-PheRS can also serve as a model for the human phenotype and possibly also for obesity.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
08 Faculty of Science > Department of Biology > Institute of Cell Biology |
UniBE Contributor: |
Brunssen, Dominique Yvonne, Suter, Beat (A) |
Subjects: |
500 Science > 570 Life sciences; biology |
ISSN: |
1933-6942 |
Publisher: |
Taylor & Francis |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
21 Feb 2024 11:00 |
Last Modified: |
21 Feb 2024 11:09 |
Publisher DOI: |
10.1080/19336934.2024.2308737 |
PubMed ID: |
38374657 |
Uncontrolled Keywords: |
Drosophila developmental delay growth roaming satiety signaling |
BORIS DOI: |
10.48350/193078 |
URI: |
https://boris.unibe.ch/id/eprint/193078 |
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