Dawson, Kara L D; Rosato, Giuliana; Egloff, Simone; Burgener, Carole; Oevermann, Anna; Grest, Paula; Hilbe, Monika; Seuberlich, Torsten (2024). Fatal tick-borne encephalitis virus infection in Dalmatian puppy-dogs after putative vector independent transmission. The veterinary quarterly, 44(1), pp. 1-7. Taylor & Francis 10.1080/01652176.2024.2338385
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Fatal_tick-borne_encephalitis_virus_infection_in_Dalmatian_puppy-dogs_after_putative_vector_independent_transmission.pdf - Published Version Available under License Creative Commons: Attribution (CC-BY). Download (8MB) | Preview |
In a retrospective metatranscriptomics study, we identified tick-borne encephalitis virus (TBEV) to be the causative agent for a fatal non-suppurative meningoencephalitis in a three-week-old Dalmatian puppy in Switzerland. Further investigations showed that the two other littermates with similar signs and pathological lesions were also positive for TBEV. By using an unbiased approach of combining high-throughput sequencing (HTS) and bioinformatics we were able to solve the etiology and discover an unusual case of TBEV in three young puppies. Based on our findings, we suggest that a vector-independent transmission of TBEV occurred and that most likely an intrauterine infection led to the severe and fulminant disease of the entire litter. We were able to demonstrate the presence of TBEV RNA by in situ hybridization (ISH) in the brain of all three puppies. Furthermore, we were able to detect TBEV by RT-qPCR in total RNA extracted from formalin-fixed and paraffin embedded (FFPE) blocks containing multiple peripheral organs. Overall, our findings shed light on alternative vector-independent transmission routes of TBEV infections in dogs and encourage veterinary practitioners to consider TBEV as an important differential diagnosis in neurological cases in dogs.
Item Type: |
Journal Article (Further Contribution) |
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Division/Institute: |
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Experimental Clinical Research 05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) |
UniBE Contributor: |
Dawson, Kara Lynne Dianne, Oevermann, Anna, Seuberlich, Torsten |
Subjects: |
600 Technology > 630 Agriculture |
ISSN: |
1875-5941 |
Publisher: |
Taylor & Francis |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
10 Apr 2024 15:32 |
Last Modified: |
10 Apr 2024 15:41 |
Publisher DOI: |
10.1080/01652176.2024.2338385 |
PubMed ID: |
38596900 |
Uncontrolled Keywords: |
Tick-borne encephalitis virus high-throughput sequencing metatranscriptomics puppies |
BORIS DOI: |
10.48350/195849 |
URI: |
https://boris.unibe.ch/id/eprint/195849 |
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