The use of light- and electron microscopy for studies on the cell- and molecular biology of parasites and parasitic diseases

Hehl, A B; Hemphill, A (2006). The use of light- and electron microscopy for studies on the cell- and molecular biology of parasites and parasitic diseases. Schweizer Archiv für Tierheilkunde, 148(9), pp. 473-81. Bern: Huber 10.1024/0036-7281.148.9.473

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Lightmicroscopical (LM) and electron microscopi cal (EM) techniques, have had a major influence on the development and direction of cell biology, and particularly also on the investigation of complex host-parasite relationships. Earlier, microscopy has been rather descriptive, but new technical and scientific advances have changed the situation. Microscopy has now become analytical, quantitative and three-dimensional, with greater emphasis on analysis of live cells with fluorescent markers. The new or improved techniques that have become available include immunocytochemistry using immunogold labeling techniques or fluorescent probes, cryopreservation and cryosectioning, in situ hybridization, fluorescent reporters for subcellular localization, micro-analytical methods for elemental distribution, confocal laser scanning microscopy, scanning tunneling microscopy and live-imaging. Taken together, these tools are providing both researchers and students with a novel and multidimensional view of the intricate biological processes during parasite development in the host.

Item Type:	Journal Article (Original Article)
Division/Institute:	05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Parasitology
UniBE Contributor:	Hemphill, Andrew
ISSN:	0036-7281
Publisher:	Huber
Language:	English
Submitter:	Factscience Import
Date Deposited:	04 Oct 2013 14:47
Last Modified:	05 Dec 2022 14:14
Publisher DOI:	10.1024/0036-7281.148.9.473
PubMed ID:	17024976
Web of Science ID:	000241096500005
URI:	https://boris.unibe.ch/id/eprint/19725 (FactScience: 2652)