Bellon, Benjamin; Pippenger, Benjamin; Stähli, Alexandra; Degen, Martin; Parisi, Ludovica (2024). Cementum and enamel surface mimicry influences soft tissue cell behavior. (In Press). Journal of periodontal research Wiley 10.1111/jre.13295
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J_of_Periodontal_Research_-_2024_-_Bellon_-_Cementum_and_enamel_surface_mimicry_influences_soft_tissue_cell_behavior.pdf - Published Version Available under License Creative Commons: Attribution-Noncommercial-No Derivative Works (CC-BY-NC-ND). Download (3MB) | Preview |
AIMS
To test whether titanium surface roughness disparity might be used to specifically guide the behavior of gingiva fibroblasts and keratinocytes, thereby improving the quality of soft tissue (ST) integration around abutments.
METHODS
Titanium discs resembling the roughness of enamel (M) or cementum (MA) were created with normal or increased hydrophilicity and used as substrates for human fibroblasts and keratinocytes. Adhesion and proliferation assays were performed to assess cell-type specific responses upon encountering the different surfaces. Additionally, immunofluorescence and qPCR analyses were performed to study more in depth the behavior of fibroblasts and keratinocytes on MA and M surfaces, respectively.
RESULTS
While enamel-like M surfaces supported adhesion, growth and a normal differentiation potential of keratinocytes, cementum-emulating MA surfaces specifically impaired the growth of keratinocytes. Vice versa, MA surfaces sustained regular adhesion and proliferation of fibroblasts. Yet, a more intimate adhesion between fibroblasts and titanium was achieved by an increased hydrophilicity of MA surfaces, which was associated with an increased expression of elastin.
CONCLUSION
The optimal titanium implant abutment might be achieved by a bimodal roughness design, mimicking the roughness of enamel (M) and cementum with increased hydrophilicity (hMA), respectively. These surfaces can selectively elicit cell responses favoring proper ST barrier by impairing epithelial downgrowth and promoting firm adhesion of fibroblasts.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
04 Faculty of Medicine > School of Dental Medicine > Department of Orthodontics 04 Faculty of Medicine > School of Dental Medicine > Orthodontic Research 04 Faculty of Medicine > School of Dental Medicine > Department of Periodontology |
UniBE Contributor: |
Pippenger, Benjamin Evans, Stähli, Alexandra Beatrice, Degen, Martin, Parisi, Ludovica |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
1600-0765 |
Publisher: |
Wiley |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
04 Jun 2024 10:04 |
Last Modified: |
05 Jun 2024 06:30 |
Publisher DOI: |
10.1111/jre.13295 |
PubMed ID: |
38828886 |
Uncontrolled Keywords: |
dental abutments fibroblasts hydrophilicity keratinocytes roughness soft tissue integration |
BORIS DOI: |
10.48350/197530 |
URI: |
https://boris.unibe.ch/id/eprint/197530 |
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