Engineering a microparticle-loaded rough membrane for guided bone regeneration modulating osteoblast response without inducing inflammation.

Díez-Tercero, Leire; Bosch-Rué, Èlia; Bosch, Begoña M; Rojas-Márquez, Raquel; Caballé-Serrano, Jordi; Delgado, Luis M; Pérez, Román A (2024). Engineering a microparticle-loaded rough membrane for guided bone regeneration modulating osteoblast response without inducing inflammation. Colloids and surfaces. B, Biointerfaces, 241(113994), p. 113994. Elsevier 10.1016/j.colsurfb.2024.113994

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Guided bone regeneration (GBR) is a widely used procedure that prevents the fast in-growth of soft tissues into bone defect. Among the different types of membranes, the use of collagen membranes is the gold standard. However, these membranes are implanted in tissue location where a severe acute inflammation will occur and can be negatively affected. The aim of this study was to develop a collagen-based membrane for GBR that incorporated alginate-hydroxyapatite microparticles. Membranes were manufactured using collagen type I and gelatin and alginate-hydroxyapatite microparticles. Membranes were assessed in terms of topography by scanning electron microscopy and confocal microscopy; stability by swelling after an overnight incubation in saline and enzymatic degradation against collagenase and mechanical properties by tensile tests. Furthermore, the biological response was assessed with SaOs-2 cells and THP-1 macrophages to determine alkaline phosphatase activity and inflammatory cytokine release. Our results showed that the incorporation of different percentages of these microparticles could induce changes in the surface topography. When the biological response was analyzed, either membranes were not cytotoxic to THP-1 macrophages or to SaOs-2 cells and they did not induce the release of pro-inflammatory cytokines. However, the different surface topographies did not induce changes in the macrophage morphology and the release of pro- and anti-inflammatory cytokines, suggesting that the effect of surface roughness on macrophage behavior could be dependent on other factors such as substrate stiffness and composition. Collagen-gelatin membranes with embedded alginate-hydroxyapatite microparticles increased ALP activity, suggesting a positive effect of them on bone regeneration, remaining unaffected the release of pro- and anti-inflammatory cytokines.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > School of Dental Medicine > Department of Periodontology

UniBE Contributor:

Caballé Serrano, Jordi

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1873-4367

Publisher:

Elsevier

Language:

English

Submitter:

Pubmed Import

Date Deposited:

12 Jun 2024 14:18

Last Modified:

29 Jul 2024 00:14

Publisher DOI:

10.1016/j.colsurfb.2024.113994

PubMed ID:

38850744

Uncontrolled Keywords:

Collagen Guided bone regeneration Macrophage Microparticle Osteoblast Surface topography

BORIS DOI:

10.48350/197699

URI:

https://boris.unibe.ch/id/eprint/197699

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