Pereira, Camila Faria de Amorim; Melo, Michelle Nonato de Oliveira; de Campos, Vania Emerich Bucco; Pereira, Ivania Paiva; Oliveira, Adriana Passos; Rocha, Mariana Souza; Batista, João Vitor da Costa; Paes de Almeida, Valter; Monchak, Irailson Thierry; Ricci-Júnior, Eduardo; Garrett, Rafael; Carvalho, Aline Gabrielle Alves; Manfron, Jane; Baumgartner, Stephan; Holandino, Carla (2024). Self-Nanoemulsifying Drug Delivery System (SNEDDS) Using Lipophilic Extract of Viscum album subsp. austriacum (Wiesb.) Vollm. International journal of nanomedicine, 19, pp. 5953-5972. DOVE Medical Press 10.2147/IJN.S464508
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IJN-464508-self-nanoemulsifying-drug-delivery-system--snedds--using-lip.pdf - Published Version Available under License Creative Commons: Attribution-Noncommercial (CC-BY-NC). Download (12MB) | Preview |
BACKGROUND AND PURPOSE
Natural products are potential sources of anticancer components. Among various species, the lipophilic extract of the Viscum album subsp. austriacum (Wiesb.) Vollm. (VALE) has shown promising therapeutic potential. The present work aimed to qualify the plant source and characterize the extract's chemical profile. In addition, a self-nanoemulsifying drug delivery system (SNEDDS) containing VALE (SNEDDS-VALE) was developed.
METHODS
V. album subsp. austriacum histochemistry was performed, and the chemical profile of VALE was analyzed by GC-MS. After the SNEEDS-VALE development, its morphology was visualized by transmission electron microscopy (TEM), while its stability was evaluated by the average droplet size, polydispersity index (PdI) and pH. Lastly, SNEDDS-VALE chemical stability was evaluated by LC-DAD-MS.
RESULTS
The histochemical analysis showed the presence of lipophilic compounds in the leaves and stems. The major compound in the VALE was oleanolic acid, followed by lupeol acetate and ursolic acid. SNEDDS was composed of medium chain triglyceride and Kolliphor® RH 40 (PEG-40 hydrogenated castor oil). A homogeneous, isotropic and stable nanoemulsion was obtained, with an average size of 36.87 ± 1.04 nm and PdI of 0.14 ± 0.02, for 14 weeks.
CONCLUSION
This is the first histochemistry analysis of V. album subsp. austriacum growing on Pinus sylvestris L. which provided detailed information regarding its lipophilic compounds. A homogeneous, isotropic and stable SNEDDS-VALE was obtained to improve the low water solubility of VALE. Further, in vitro and in vivo experiments should be performed, in order to evaluate the antitumoral potential of SNEDDS-VALE.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > Medical Education > Institute of Complementary and Integrative Medicine (IKIM) |
UniBE Contributor: |
Baumgartner, Stephan |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
1176-9114 |
Publisher: |
DOVE Medical Press |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
20 Jun 2024 14:40 |
Last Modified: |
22 Jun 2024 03:08 |
Publisher DOI: |
10.2147/IJN.S464508 |
PubMed ID: |
38895147 |
Uncontrolled Keywords: |
SNEDDS Viscum album subsp. austriacum lipophilic extract mistletoe oleanolic acid |
BORIS DOI: |
10.48350/197946 |
URI: |
https://boris.unibe.ch/id/eprint/197946 |
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