Identification of two autoantigens recognised by circulating autoantibodies as potential biomarkers for diagnosing giant cell arteritis.

Pesce, Elisa; Bombaci, Mauro; Croci, Stefania; Bonacini, Martina; Marvisi, Chiara; Ricordi, Caterina; Monti, Sara; Muratore, Francesco; Abrignani, Sergio; Caporali, Roberto; Borghi, Maria Orietta; Salvarani, Carlo; Villiger, Peter M; Grifantini, Renata; Meroni, Pier Luigi (2024). Identification of two autoantigens recognised by circulating autoantibodies as potential biomarkers for diagnosing giant cell arteritis. Clinical and experimental rheumatology, 42(7), pp. 1317-1320. Pacini editore 10.55563/clinexprheumatol/0213qf

[img] Text
article.pdf - Published Version
Restricted to registered users only
Available under License Publisher holds Copyright.

Download (552kB)

OBJECTIVES

Giant cell arteritis (GCA) is a common vasculitis affecting patients aged 50 and older. GCA leads to chronic inflammation of large/medium-sized vessel walls with complications such as permanent vision loss and risk of stroke and aortic aneurysms. Early diagnosis is crucial and relies on temporal artery biopsy (TAB) and ultrasound imaging of temporal and axillary arteries. However, these methods have limitations. Serum biomarkers as autoantibodies have been reported but with inconclusive data for their use in the clinical setting. Additionally, C-reactive protein and erythrocyte sedimentation rate are non-specific and limited in reflecting disease activity, particularly in patients treated with IL-6 inhibitors. This study aimed to identify serum autoantibodies as new diagnostic biomarkers for GCA using a human protein array.

METHODS

One commercial and one proprietary human protein array were used for antibody profiling of sera from patients with GCA (n=55), Takayasu (TAK n=7), and Healthy Controls (HC n=28). The identified candidate autoantigens were purified and tested for specific autoantibodies by ELISA.

RESULTS

Antibodies against two proteins, VSIG10L (V-Set and Immunoglobulin Domain Containing 10 Like) and DCBLD1 (discoidin), were identified and found to be associated with GCA, with an overall prevalence of 43-57%, respectively, and high specificity as individual antibodies. A control series of TAK sera tested negative.

CONCLUSIONS

Detecting GCA-specific autoantibodies may offer a new, non-invasive tool for improving our diagnostic power in GCA. Even though cell-mediated immune responses are crucial for GCA pathogenesis, this finding opens the way for investigating the additional role of humoral immune responses in the disease.

Item Type:

Journal Article (Original Article)

UniBE Contributor:

Villiger, Peter Matthias

Subjects:

600 Technology > 610 Medicine & health

ISSN:

0392-856X

Publisher:

Pacini editore

Language:

English

Submitter:

Pubmed Import

Date Deposited:

09 Jul 2024 10:39

Last Modified:

20 Jul 2024 00:16

Publisher DOI:

10.55563/clinexprheumatol/0213qf

PubMed ID:

38976303

BORIS DOI:

10.48350/198693

URI:

https://boris.unibe.ch/id/eprint/198693

Actions (login required)

Edit item Edit item
Provide Feedback