Gene expression by human monocytes from peripheral blood in response to exposure to metals

Jost-Albrecht, Katrin; Hofstetter, Willy (2006). Gene expression by human monocytes from peripheral blood in response to exposure to metals. Journal of biomedical materials research. Part B - applied biomaterials, 76(2), pp. 449-55. Hoboken, N.J.: John Wiley & Sons 10.1002/jbm.b.30389

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With increasing life expectancy and active lifestyles, the longevity of arthroplasties has become an important problem in orthopaedic surgery and will remain so until novel approaches to joint preservation have been developed. The sensitivity of the recipient to the metal alloys may be one of the factors limiting the lifespan of implants. In the present study, the response of human monocytes from peripheral blood to an exposure to metal ions was investigated, using the method of real-time polymerase chain reaction (PCR)-based low-density arrays. Upon stimulation with bivalent (Co2+ and Ni2+) and trivalent (Ti3+) cations and with the calcium antagonist LaCl3, the strength of the elicited monocytic response was in the order of Co2+ > or = Ni2+ > Ti3+ > or = LaCl3. The transcriptional regulation of the majority of genes affected by the exposure of monocytes to Co2+ and Ni2+ was similar. Some genes critically involved in the processes of inflammation and bone resorption, however, were found to be differentially regulated by these bivalent cations. The data demonstrate that monocytic gene expression is adapted in response to metal ions and that this response is, in part, specific for the individual metals. It is suggested that metal alloys used in arthroplasties may affect the extent of inflammation and bone resorption in the peri-implant tissues in dependence of their chemical composition.

Item Type:	Journal Article (Original Article)
Division/Institute:	04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Knochenbiologie & Orthopädische Forschung 04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Knochenbiologie & Orthopädische Forschung
UniBE Contributor:	Hofstetter, Wilhelm (B)
ISSN:	1552-4973
ISBN:	16211567
Publisher:	John Wiley & Sons
Language:	English
Submitter:	Factscience Import
Date Deposited:	04 Oct 2013 14:48
Last Modified:	02 Mar 2023 23:22
Publisher DOI:	10.1002/jbm.b.30389
PubMed ID:	16211567
Web of Science ID:	000234834700027
URI:	https://boris.unibe.ch/id/eprint/19912 (FactScience: 2989)