Ammann, Roland A; Zucol, Franziska; Aebi, Christoph; Niggli, Felix K; Kühne, Thomas; Nadal, David (2007). Real-time broad-range PCR versus blood culture. A prospective pilot study in pediatric cancer patients with fever and neutropenia. Supportive care in cancer, 15(6), pp. 637-41. Berlin: Springer-Verlag 10.1007/s00520-007-0235-x
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MATERIALS AND METHODS: In a pilot study, results of real-time broad-range (16S rRNA) polymerase chain reaction (PCR) performed on 45 blood samples of pediatric cancer patients with fever and neutropenia were compared with blood culture results. RESULTS: The PCR assay used, having proven a high sensitivity in artificially spiked blood samples, was positive in only three of ten blood culture-positive samples, and it was positive in 10 of 35 (29%) culture-negative samples. CONCLUSION: This broad-range PCR assay, which may identify not-grown bacteria potentially contributing to fever, needs improvement in sensitivity, and different reasons for positive PCR in negative blood culture samples need to be assessed before clinical application.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > Department of Gynaecology, Paediatrics and Endocrinology (DFKE) > Clinic of Paediatric Medicine |
UniBE Contributor: |
Ammann, Roland, Aebi, Christoph |
ISSN: |
0941-4355 |
ISBN: |
17333292 |
Publisher: |
Springer-Verlag |
Language: |
English |
Submitter: |
Anette van Dorland |
Date Deposited: |
04 Oct 2013 14:55 |
Last Modified: |
05 Dec 2022 14:17 |
Publisher DOI: |
10.1007/s00520-007-0235-x |
PubMed ID: |
17333292 |
Web of Science ID: |
000247531600008 |
BORIS DOI: |
10.7892/boris.23380 |
URI: |
https://boris.unibe.ch/id/eprint/23380 (FactScience: 41660) |
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