Pfister, Frederick; Wang, Yumei; Schreiter, Kay; vom Hagen, Franziska; Altvater, Karin; Hoffmann, Sigrid; Deutsch, Urban; Hammes, Hans-Peter; Feng, Yuxi (2010). Retinal overexpression of angiopoietin-2 mimics diabetic retinopathy and enhances vascular damages in hyperglycemia. Acta diabetologica, 47(1), pp. 59-64. Milano: Springer 10.1007/s00592-009-0099-2
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Our previous data suggested that angiopoietin-2 (Ang-2) is linked to pericyte loss, thereby playing an important role in diabetic retinopathy. In this study, we investigated the effect of retinal overexpression of human Ang-2 (mOpsinhAng2 mouse) on vascular morphology in non-diabetic and streptozotozin-induced diabetic animals. Pericyte (PC) coverage and acellular capillary (AC) formation were quantitated in retinal digest preparations after 3 and 6 months of diabetes duration. The degree of retinopathy in non-diabetic mOpsinhAng2 mice at 3 months (-21% PC, +49% AC) was comparable to age-matched diabetic wild type mice. Diabetic mOpsinhAng2 mice exhibited significantly worse vascular pathology than wild type counterparts at 6 months. Quantitative PCR revealed that human Ang-2 mRNA was highly overexpressed in retinas of transgenic mice. Our data demonstrate that overexpression of Ang-2 in the retina enhances vascular pathology, indicating that Ang-2 plays an essential role in diabetic vasoregression via destabilization of pericytes.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > Pre-clinic Human Medicine > Theodor Kocher Institute |
UniBE Contributor: |
Deutsch, Urban |
ISSN: |
0940-5429 |
Publisher: |
Springer |
Language: |
English |
Submitter: |
Factscience Import |
Date Deposited: |
04 Oct 2013 14:07 |
Last Modified: |
05 Dec 2022 14:00 |
Publisher DOI: |
10.1007/s00592-009-0099-2 |
PubMed ID: |
19238311 |
Web of Science ID: |
000274267100010 |
BORIS DOI: |
10.7892/boris.244 |
URI: |
https://boris.unibe.ch/id/eprint/244 (FactScience: 197082) |
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