Different modes of translation for hid, grim and sickle mRNAs in Drosophila

Vazquez-Pianzola, Paula; Hernandez, Greco; Suter, Beat; Rivera-Pomar, Ronaldo (2007). Different modes of translation for hid, grim and sickle mRNAs in Drosophila. Cell death and differentiation, 14(2), pp. 286-295. Basingstoke: Nature Publishing Group 10.1038/sj.cdd.4401990

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Protein synthesis is inhibited during apoptosis. However, the translation of many mRNAs still proceeds driven by internal ribosome entry sites (IRESs). Here we show that the 5'UTR of hid and grim mRNAs promote translation of uncapped-mRNA reporters in cell-free embryonic extracts and that hid and grim mRNA 5'UTRs drive IRES-mediated translation. The translation of capped-reporters proceeds in the presence of cap competitor and in extracts where cap-dependent translation is impaired. We show that the endogenous hid and grim mRNAs are present in polysomes of heat-shocked embryos, indicating that cap recognition is not required for translation. In contrast, sickle mRNA is translated in a cap-dependent manner in all these assays. Our results show that IRES-dependent initiation may play a role in the translation of Drosophila proapoptotic genes and suggest a variety of regulatory pathways.

Item Type:	Journal Article (Original Article)
Division/Institute:	08 Faculty of Science > Department of Biology > Institute of Cell Biology
UniBE Contributor:	Suter, Beat (A)
ISSN:	1350-9047
Publisher:	Nature Publishing Group
Language:	English
Submitter:	Factscience Import
Date Deposited:	04 Oct 2013 14:57
Last Modified:	29 Mar 2023 23:33
Publisher DOI:	10.1038/sj.cdd.4401990
Web of Science ID:	000243493000009
URI:	https://boris.unibe.ch/id/eprint/24679 (FactScience: 52810)