Schreiber, N; Khattab, A; Petter, M; Marks, F; Adjei, S; Kobbe, R; May, J; Klinkert, M-Q (2008). Expression of Plasmodium falciparum 3D7 STEVOR proteins for evaluation of antibody responses following malaria infections in naïve infants. Parasitology, 135(2), pp. 155-67. London: Cambridge University Press 10.1017/S0031182007003794
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expression-of-plasmodium-falciparum-3d7-stevor-proteins-for-evaluation-of-antibody-responses-following-malaria-infections-in-naive-infants.pdf - Published Version Available under License Publisher holds Copyright. Download (681kB) | Preview |
Clinical immunity to Plasmodium falciparum malaria develops after repeated exposure to the parasite. At least 2 P. falciparum variant antigens encoded by multicopy gene families (var and rif) are targets of this adaptive antibody-mediated immunity. A third multigene family of variant antigens comprises the stevor genes. Here, 4 different stevor sequences were selected for cloning and expression in Escherichia coli and His6-tagged fusion proteins were used for assessing the development of immunity. In a cross-sectional analysis of clinically immune adults living in a malaria endemic area in Ghana, high levels of anti-STEVOR IgG antibody titres were determined in ELISA. A cross-sectional study of 90 nine-month-old Ghanaian infants using 1 recombinant STEVOR showed that the antibody responses correlated positively with the number of parasitaemia episodes. In a longitudinal investigation of 17 immunologically naïve 9-month-old infants, 3 different patterns of anti-STEVOR antibody responses could be distinguished (high, transient and low). Children with high anti-STEVOR-antibody levels exhibited an elevated risk for developing parasitaemia episodes. Overall, a protective effect could not be attributed to antibodies against the STEVOR proteins chosen for the study presented here.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > Department of Cardiovascular Disorders (DHGE) > Clinic of Cardiology |
UniBE Contributor: |
Khattab, Ahmed Aziz |
ISSN: |
0031-1820 |
ISBN: |
17931459 |
Publisher: |
Cambridge University Press |
Language: |
English |
Submitter: |
Factscience Import |
Date Deposited: |
04 Oct 2013 15:02 |
Last Modified: |
05 Dec 2022 14:19 |
Publisher DOI: |
10.1017/S0031182007003794 |
PubMed ID: |
17931459 |
Web of Science ID: |
000253267000002 |
BORIS DOI: |
10.48350/26970 |
URI: |
https://boris.unibe.ch/id/eprint/26970 (FactScience: 99320) |
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