Characterization of the CopR regulon of Lactococcus lactis IL1403

Magnani, David; Barré, Olivier; Gerber, Simon D; Solioz, Marc (2008). Characterization of the CopR regulon of Lactococcus lactis IL1403. Journal of bacteriology, 190(2), pp. 536-45. Washington, D.C.: American Society for Microbiology 10.1128/JB.01481-07

Full text not available from this repository.

To identify components of the copper homeostatic mechanism of Lactococcus lactis, we employed two-dimensional gel electrophoresis to detect changes in the proteome in response to copper. Three proteins upregulated by copper were identified: glyoxylase I (YaiA), a nitroreductase (YtjD), and lactate oxidase (LctO). The promoter regions of these genes feature cop boxes of consensus TACAnnTGTA, which are the binding site of CopY-type copper-responsive repressors. A genome-wide search for cop boxes revealed 28 such sequence motifs. They were tested by electrophoretic mobility shift assays for the interaction with purified CopR, the CopY-type repressor of L. lactis. Seven of the cop boxes interacted with CopR in a copper-sensitive manner. They were present in the promoter region of five genes, lctO, ytjD, copB, ydiD, and yahC; and two polycistronic operons, yahCD-yaiAB and copRZA. Induction of these genes by copper was confirmed by real-time quantitative PCR. The copRZA operon encodes the CopR repressor of the regulon; a copper chaperone, CopZ; and a putative copper ATPase, CopA. When expressed in Escherichia coli, the copRZA operon conferred copper resistance, suggesting that it functions in copper export from the cytoplasm. Other member genes of the CopR regulon may similarly be involved in copper metabolism.

Item Type:	Journal Article (Original Article)
Division/Institute:	04 Faculty of Medicine > Service Sector > Institute of Clinical Pharmacology and Visceral Research [discontinued] 04 Faculty of Medicine > Department of Dermatology, Urology, Rheumatology, Nephrology, Osteoporosis (DURN) > Clinic of Rheumatology and Immunology
UniBE Contributor:	Barré, Olivier, Gerber, Simon (B), Solioz, Marc
ISSN:	0021-9193
ISBN:	17993525
Publisher:	American Society for Microbiology
Language:	English
Submitter:	Factscience Import
Date Deposited:	04 Oct 2013 15:04
Last Modified:	29 Mar 2023 23:33
Publisher DOI:	10.1128/JB.01481-07
PubMed ID:	17993525
Web of Science ID:	000252392100008
URI:	https://boris.unibe.ch/id/eprint/27638 (FactScience: 109754)