Effects of four-week high-fructose diet on gene expression in skeletal muscle of healthy men

Lê, K-A; Faeh, D; Stettler, R; Debard, C; Loizon, E; Vidal, H; Boesch, C; Ravussin, E; Tappy, L (2008). Effects of four-week high-fructose diet on gene expression in skeletal muscle of healthy men. Diabetes & metabolism, 34(1), pp. 82-5. Issy-les-Moulineaux (F): Elsevier Masson SAS 10.1016/j.diabet.2007.08.004

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AIMS: A high-fructose diet (HFrD) may play a role in the obesity and metabolic disorders epidemic. In rodents, HFrD leads to insulin resistance and ectopic lipid deposition. In healthy humans, a four-week HFrD alters lipid homoeostasis, but does not affect insulin sensitivity or intramyocellular lipids (IMCL). The aim of this study was to investigate whether fructose may induce early molecular changes in skeletal muscle prior to the development of whole-body insulin resistance. METHODS: Muscle biopsies were taken from five healthy men who had participated in a previous four-week HFrD study, during which insulin sensitivity (hyperinsulinaemic euglycaemic clamp), and intrahepatocellular lipids and IMCL were assessed before and after HFrD. The mRNA concentrations of 16 genes involved in lipid and carbohydrate metabolism were quantified before and after HFrD by real-time quantitative PCR. RESULTS: HFrD significantly (P<0.05) increased stearoyl-CoA desaturase-1 (SCD-1) (+50%). Glucose transporter-4 (GLUT-4) decreased by 27% and acetyl-CoA carboxylase-2 decreased by 48%. A trend toward decreased peroxisomal proliferator-activated receptor-gamma coactivator-1alpha (PGC-1alpha) was observed (-26%, P=0.06). All other genes showed no significant changes. CONCLUSION: HFrD led to alterations of SCD-1, GLUT-4 and PGC-1alpha, which may be early markers of insulin resistance.

Item Type:	Journal Article (Original Article)
Division/Institute:	04 Faculty of Medicine > Department of Radiology, Neuroradiology and Nuclear Medicine (DRNN) > Institute of Diagnostic, Interventional and Paediatric Radiology > DCR Magnetic Resonance Spectroscopy and Methodology (AMSM)
UniBE Contributor:	Boesch, Christoph Hans
ISSN:	1262-3636
ISBN:	18063403
Publisher:	Elsevier Masson SAS
Language:	English
Submitter:	Factscience Import
Date Deposited:	04 Oct 2013 15:06
Last Modified:	02 Mar 2023 23:23
Publisher DOI:	10.1016/j.diabet.2007.08.004
PubMed ID:	18063403
Web of Science ID:	000253771500013
URI:	https://boris.unibe.ch/id/eprint/28534 (FactScience: 121222)