Obukhova, Polina; Rieben, Robert; Bovin, Nicolai (2007). Normal human serum contains high levels of anti-Gal alpha 1-4GlcNAc antibodies. Xenotransplantation, 14(6), pp. 627-35. Hoboken, N.J.: Wiley 10.1111/j.1399-3089.2007.00436.x
Full text not available from this repository. (Request a copy)BACKGROUND: Natural xenoreactive antibodies (Abs) directed against the Bdi-epitope (Gal alpha 1-3Gal beta) on the cells of non-primate mammals take part in hyperacute rejection of xenotransplanted organs. We found that some Abs, which were one-step affinity purified on Bdi-Sepharose, cross-reacted with the disaccharide Gal alpha 1-4GlcNAc beta. The epitope Gal alpha 1-4GlcNAc has not been identified on mammals or bacterial polysaccharides yet. METHODS: To isolate the antibodies of the corresponding specificity the disaccharide was immobilized on Sepharose and antibodies were affinity purified from pooled serum of blood group O individuals. RESULTS: These one-step purified Abs cross-reacted with Bdi, but after a prior absorption step on Bdi-Sepharose no cross-reactivity with Bdi was observed any longer. Surprisingly, the quantity of anti-Gal alpha 1-4GlcNAc isolated from the same serum pool, 4-7 microg/ml, was equal to that of anti-Bdi or more. Independently of ABO blood groups all the tested healthy donors had anti-Gal alpha 1-4GlcNAc Abs at a similar level. Monospecific anti-Gal alpha 1-4GlcNAc Abs were not cytotoxic towards porcine cells. CONCLUSIONS: 1. The actual concentration of monospecific, xenoreactive Gal alpha 1-3Gal beta Abs in blood may be considerably lower than the value referred to in the literature for 'anti-alpha Gal' or 'anti-Galili' antibodies. 2. Anti-Gal alpha 1-4GlcNAc Abs seem not to be important for xenotransplantation.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Forschungsbereich Mu50 > Forschungsgruppe Herz und Gefässe |
UniBE Contributor: |
Rieben, Robert |
ISSN: |
0908-665X |
ISBN: |
17991151 |
Publisher: |
Wiley |
Language: |
English |
Submitter: |
Factscience Import |
Date Deposited: |
04 Oct 2013 15:09 |
Last Modified: |
05 Dec 2022 14:21 |
Publisher DOI: |
10.1111/j.1399-3089.2007.00436.x |
PubMed ID: |
17991151 |
Web of Science ID: |
000250766000008 |
URI: |
https://boris.unibe.ch/id/eprint/30168 (FactScience: 186483) |
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