Tappeiner, Christoph; Goldblum, David; Katsoulis, Konstantinos; Sarra, Gian-Marco; Früh Epstein, Beatrice (2011). In vivo measurement of central corneal thickness in normal chicks (Gallus gallus domesticus) with the optical low-coherence reflectometer. Veterinary ophthalmology, 14(4), pp. 257-261. Gainesville, Fla.: Blackwell Science 10.1111/j.1463-5224.2011.00875.x
Full text not available from this repository.OBJECTIVE
To determine the practicability and accuracy of central corneal thickness (CCT) measurements in living chicks utilizing a noncontact, high-speed optical low-coherence reflectometer (OLCR) mounted on a slit lamp.
ANIMALS STUDIED
Twelve male chicks (Gallus gallus domesticus). Procedures Measurements of CCT were obtained in triplicate in 24 eyes of twelve 1-day-old anaesthetized chicks using OLCR. Every single measurement taken by OLCR consisted of the average result of 20 scans obtained within seconds. Additionally, corneal thickness was determined histologically after immersion fixation in Karnovsky's solution alone (20 eyes) or with a previous injection of the fixative into the anterior chamber before enucleation (4 eyes).
RESULTS
Central corneal thickness measurements using OLCR in 1-day-old living chicks provide a rapid and feasible examination technique. Mean CCT measured with OLCR (189.7 ± 3.34 μm) was significantly lower than histological measurements (242.1 ± 47.27 μm) in eyes with fixation in Karnovsky's solution (P = 0.0005). In eyes with additional injection of Karnovsky's fixative into the anterior chamber, mean histologically determined CCT was 195.2 ± 8.25 μm vs. 191.9 ± 8.90 μm with OLCR. A trend for a lower variance was found compared to the eyes that had only been immersion fixed.
CONCLUSION
Optical low-coherence reflectometry is an accurate examination technique to measure in vivo CCT in the eye of newborn chicks. The knowledge of the thickness of the chick cornea and the ability to obtain noninvasive, noncontact measurements of CCT in the living animal may be of interest for research and development of eye diseases in chick models.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > Department of Head Organs and Neurology (DKNS) > Clinic of Ophthalmology 04 Faculty of Medicine > Department of Head Organs and Neurology (DKNS) > Clinic of Craniomaxillofacial Surgery |
UniBE Contributor: |
Tappeiner, Christoph, Goldblum, David, Katsoulis, Konstantinos, Sarra, Gian-Marco, Früh Epstein, Beatrice |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
1463-5216 |
Publisher: |
Blackwell Science |
Language: |
English |
Submitter: |
Christoph Tappeiner |
Date Deposited: |
04 Oct 2013 14:14 |
Last Modified: |
05 Dec 2022 14:03 |
Publisher DOI: |
10.1111/j.1463-5224.2011.00875.x |
PubMed ID: |
21733067 |
Web of Science ID: |
000292514900006 |
URI: |
https://boris.unibe.ch/id/eprint/3548 (FactScience: 207410) |
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