Molecular epidemiology and genetic linkage of macrolide and aminoglycoside resistance in Staphylococcus intermedius of canine origin

Boerlin, P.; Burnens, A. P.; Frey, Joachim; Kuhnert, Peter; Nicolet, J. (2001). Molecular epidemiology and genetic linkage of macrolide and aminoglycoside resistance in Staphylococcus intermedius of canine origin. Veterinary microbiology, 79(2), pp. 155-169. Amsterdam: Elsevier 10.1016/S0378-1135(00)00347-3

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A collection of 77 Staphylococcus intermedius isolates from dogs and cats in Switzerland was examined for resistance to erythromycin. Resistance profiles for 14 additional antibiotics were compared between erythromycin-resistant and susceptible isolates. A resistance prevalence of 27% for erythromycin was observed in the population under study. Complete correlation between resistance to erythromycin, and to spiramycin, streptomycin, and neomycin was observed. The erythromycin-resistant isolates all had a reduced susceptibility to clindamycin when compared to the erythromycin-susceptible isolates. Both constitutive and inducible resistance phenotypes were observed for clindamycin. Ribotyping showed that macrolide-aminoglycoside resistance was randomly distributed among unrelated strains. This suggests that this particular resistance profile is not related to a single bacterial clone but to the horizontal transfer of resistance gene clusters in S. intermedius populations. The erythromycin-resistant isolates were all carrying erm(B), but not erm(A), erm(C), or msr(A). The erm(B) gene was physically linked to Tn5405-like elements known as resistance determinants for streptomycin, streptothricin, neomycin and kanamycin. Analysis of the region flanking erm(B) showed the presence of two different groups of erm(B)-Tn5405-like elements in the S. intermedius population examined and of elements found in Gram-positive species other than staphylococci. This strongly suggests that erm(B) or the whole erm(B)-Tn5405-like elements in S. intermedius originate from other bacterial species, possibly from enterococci.

Item Type:	Journal Article (Original Article)
Division/Institute:	05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology
UniBE Contributor:	Frey, Joachim, Kuhnert, Peter
Subjects:	500 Science > 570 Life sciences; biology 600 Technology > 630 Agriculture
ISSN:	0378-1135
Publisher:	Elsevier
Language:	English
Submitter:	Peter Kuhnert-Ryser
Date Deposited:	05 Jun 2014 16:06
Last Modified:	05 Dec 2022 14:27
Publisher DOI:	10.1016/S0378-1135(00)00347-3
PubMed ID:	11230937
URI:	https://boris.unibe.ch/id/eprint/39153