Meier, Pascal; Glökler, Steffen; Oezdemir, Berna; Indermuehle, Andreas; Traupe, Tobias; Vogel, Rolf; de Marchi, Stefano; Seiler, Christian (2013). G-CSF induced arteriogenesis in humans: molecular insights into a randomized controlled trial. Current vascular pharmacology, 11(1), pp. 38-46. Bentham Science Publishers 10.2174/157016113804547674
Full text not available from this repository. (Request a copy)AIMS
Recent data have demonstrated the feasibility of therapeutic induction of coronary collateral growth (arteriogenesis); however, mechanisms of action of such therapeutic collateral stimulation in humans are unknown. The aim of this study was to evaluate potential mechanisms, especially the involvement of arteriogenesis-relevant genes.
METHODS AND RESULTS
A total of 52 patients were randomized into two groups: subcutaneous G-CSF (10 μg/kg; n=26) or placebo (n=26). Before and after this 2-week treatment, collateral-flow index (CFI) was determined by simultaneous measurement of mean aortic, distal coronary occlusive and central venous pressure. CD34+ endothelial progenitor cells (EPC) and monocytes were quantified before, during and after treatment; gene-expression analysis of monocytes was performed with real-time polymerase chain reaction (RT-PCR). G-CSF lead to a significant increase of EPC and monocytes (4.8 and 2.6 fold, p < 0.05); for both cell types, the extent of increase correlated with CFI increase (r=0.23 and 0.14, p < 0.05). G-CSF also induced a change in gene expression of pro-and anti-arteriogenic genes in monocytes. Among nine assessed genes, three were found to be differentially regulated (IL8, JAK2, and PNPLa4; p < 0.05).
CONCLUSIONS
The mechanism of induction of collateral growth by G-CSF is related to an increase of EPC and of peripheral monocytes. It also leads to a change toward a pro-arteriogenic gene expression in peripheral monocytes.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > Department of Cardiovascular Disorders (DHGE) > Clinic of Cardiology |
UniBE Contributor: |
Glökler, Steffen, Traupe, Tobias, De Marchi, Stefano, Seiler, Christian |
Subjects: |
600 Technology > 610 Medicine & health 500 Science > 570 Life sciences; biology |
ISSN: |
1570-1611 |
Publisher: |
Bentham Science Publishers |
Language: |
English |
Submitter: |
PD Dr. Tobias Traupe |
Date Deposited: |
19 Mar 2014 13:42 |
Last Modified: |
02 Mar 2023 23:24 |
Publisher DOI: |
10.2174/157016113804547674 |
PubMed ID: |
23391421 |
URI: |
https://boris.unibe.ch/id/eprint/43356 |
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