Küng, Eliane; Frey, Joachim (2013). AvxA, a composite serine-protease-RTX toxin of Avibacterium paragallinarum. Veterinary microbiology, 163(3-4), pp. 290-298. Elsevier 10.1016/j.vetmic.2012.12.029
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Avibacterium paragallinarum, the etiological agent of infectious coryza in chicken, was found to encode a bivalent serine-protease - RTX-porin toxin named AvxA. This toxin is encoded on a classical RTX operon structure with the activator gene avxC, the structural serin-protease-RTX toxin gene avxA, and the genes for a proper type I secretion system avxBD. AvxA is activated by the product of the avxC gene, secreted by the avxBD specified type I secretion system and proteolytically processed leaving a 95 kDa RTX moiety that is found in culture supernatants of A. paragallinarum serovars A, B and C. The RTX moiety of AvxA (AvxA-RTX) is cytotoxic against the avian macrophage like cell line HD11 but not against bovine macrophage cell line BoMac. Purified IgG from hyper-immune rabbit anti-AvxA-RTX serum made by immunization with recombinant AvxA-RTX from a serotype A strain fully neutralizes the cytotoxic activity of recombinant active AvxA-RTX and of A. paragallinarum serotypes A, B and C. This indicates that AvxA is a common major virulence attribute of all A. paragallinarum serotypes.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction 05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) 05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology |
UniBE Contributor: |
Frey, Joachim |
Subjects: |
600 Technology > 630 Agriculture |
ISSN: |
0378-1135 |
Publisher: |
Elsevier |
Language: |
English |
Submitter: |
Susanne Portner |
Date Deposited: |
22 Aug 2014 12:10 |
Last Modified: |
05 Dec 2022 14:29 |
Publisher DOI: |
10.1016/j.vetmic.2012.12.029 |
PubMed ID: |
23380459 |
Uncontrolled Keywords: |
Avian coryza, RTX toxin, Cytotoxicity, Avian macrophage like cells, Serine protease, Host specificity |
BORIS DOI: |
10.7892/boris.43812 |
URI: |
https://boris.unibe.ch/id/eprint/43812 |
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