Debache, Karim; Hemphill, Andrew (2013). Differential effects of intranasal vaccination with recombinant NcPDI in different mouse models of Neospora caninum infection. Parasite immunology, 35(1), pp. 11-20. Blackwell Scientific Publications 10.1111/pim.12013
Full text not available from this repository. (Request a copy)In this study, mice were vaccinated intranasally with recombinant N. caninum protein disulphide isomerase (NcPDI) emulsified in cholera toxin (CT) or cholera toxin subunit B (CTB) from Vibrio cholerae. The effects of vaccination were assessed in the murine nonpregnant model and the foetal infection model, respectively. In the nonpregnant mice, previous results were confirmed, in that intranasal vaccination with recNcPDI in CT was highly protective, and low cerebral parasite loads were noted upon real-time PCR analysis. Protection was accompanied by an IgG1-biased anti-NcPDI response upon infection and significantly increased expression of Th2 (IL-4/IL-10) and IL-17 transcripts in spleen compared with corresponding values in mice treated with CT only. However, vaccination with recNcPDI in CT did not induce significant protection in dams and their offspring. In the dams, increased splenic Th1 (IFN-γ/IL-12) and Th17 mRNA expressions was detected. No protection was noted in the groups vaccinated with recNcPDI emulsified in CTB. Thus, vaccination with recNcPDI in CT in nonpregnant mice followed by challenge infection induced a protective Th2-biased immune response, while in the pregnant mouse model, the same vaccine formulation resulted in a Th1-biased inflammatory response and failed to protect dams and their progeny.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction 05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Parasitology 05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) |
UniBE Contributor: |
Debache, Karim, Hemphill, Andrew |
Subjects: |
600 Technology > 630 Agriculture |
ISSN: |
0141-9838 |
Publisher: |
Blackwell Scientific Publications |
Language: |
English |
Submitter: |
Susanne Portner |
Date Deposited: |
28 Jul 2014 12:17 |
Last Modified: |
05 Dec 2022 14:30 |
Publisher DOI: |
10.1111/pim.12013 |
PubMed ID: |
23046358 |
Uncontrolled Keywords: |
adjuvant, enzyme-linked immunosorbent assay, Neospora caninum, parasite, Th1, Th2, vaccination |
URI: |
https://boris.unibe.ch/id/eprint/44720 |
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