Mayer, Jürgen; Robert-Moreno, Alexandre; Danuser, Renzo; Stein, Jens Volker; Sharpe, James; Swoger, Jim (2014). OPTiSPIM: integrating optical projection tomography in light sheet microscopy extends specimen characterization to nonfluorescent contrasts. Optics letters, 39(4), pp. 1053-1056. Optical Society of America 10.1364/OL.39.001053
Full text not available from this repository.Mesoscopic 3D imaging has become a widely used optical imaging technique to visualize intact biological specimens. Selective plane illumination microscopy (SPIM) visualizes samples up to a centimeter in size with micrometer resolution by 3D data stitching but is limited to fluorescent contrast. Optical projection tomography (OPT) works with fluorescent and nonfluorescent contrasts, but its resolution is limited in large samples. We present a hybrid setup (OPTiSPIM) combining the advantages of each technique. The combination of fluorescent and nonfluorescent high-resolution 3D data into integrated datasets enables a more extensive representation of mesoscopic biological samples. The modular concept of the OPTiSPIM facilitates incorporation of the transmission OPT modality into already established light sheet based imaging setups.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > Pre-clinic Human Medicine > Theodor Kocher Institute |
UniBE Contributor: |
Stein, Jens Volker |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
0146-9592 |
Publisher: |
Optical Society of America |
Language: |
English |
Submitter: |
Ursula Zingg-Zünd |
Date Deposited: |
24 Sep 2014 16:35 |
Last Modified: |
05 Dec 2022 14:31 |
Publisher DOI: |
10.1364/OL.39.001053 |
PubMed ID: |
24562276 |
URI: |
https://boris.unibe.ch/id/eprint/46355 |
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