Establishing a fiber-optic-based optical neural interface.

Adamantidis, Antoine Roger; Zhang, Feng; de Lecea, Luis; Deisseroth, Karl (2014). Establishing a fiber-optic-based optical neural interface. Cold Spring Harbor protocols, 2014(8), pp. 839-844. Cold Spring Harbor Laboratory 10.1101/pdb.prot083337

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Selective expression of opsins in genetically defined neurons makes it possible to control a subset of neurons without affecting nearby cells and processes in the intact brain, but light must still be delivered to the target brain structure. Light scattering limits the delivery of light from the surface of the brain. For this reason, we have developed a fiber-optic-based optical neural interface (ONI), which allows optical access to any brain structure in freely moving mammals. The ONI system is constructed by modifying the small animal cannula system from PlasticsOne. The system for bilateral stimulation consists of a bilateral cannula guide that has been stereotactically implanted over the target brain region, a screw cap for securing the optical fiber to the animal's head, a fiber guard modified from the internal cannula adapter, and a bare fiber whose length is customized based on the depth of the target region. For unilateral stimulation, a single-fiber system can be constructed using unilateral cannula parts from PlasticsOne. We describe here the preparation of the bilateral ONI system and its use in optical stimulation of the mouse or rat brain. Delivery of opsin-expressing virus and implantation of the ONI may be conducted in the same surgical session; alternatively, with a transgenic animal no opsin virus is delivered during the surgery. Similar procedures are useful for deep or superficial injections (even for neocortical targets, although in some cases surface light-emitting diodes or cortex-apposed fibers can be used for the most superficial cortical targets).

Item Type:	Journal Article (Review Article)
Division/Institute:	04 Faculty of Medicine > Department of Head Organs and Neurology (DKNS) > Clinic of Neurology
UniBE Contributor:	Adamantidis, Antoine Roger
Subjects:	600 Technology > 610 Medicine & health
ISSN:	1940-3402
Publisher:	Cold Spring Harbor Laboratory
Language:	English
Submitter:	Valentina Rossetti
Date Deposited:	27 Feb 2015 10:44
Last Modified:	05 Dec 2022 14:41
Publisher DOI:	10.1101/pdb.prot083337
PubMed ID:	25086020
URI:	https://boris.unibe.ch/id/eprint/63671