de Witt, Susanne M; Swieringa, Frauke; Cavill, Rachel; Lamers, Moniek M E; van Kruchten, Roger; Mastenbroek, Tom; Baaten, Constance; Coort, Susan; Pugh, Nicholas; Schulz, Ansgar; Scharrer, Inge; Jurk, Kerstin; Zieger, Barbara; Clemetson, Kenneth John; Farndale, Richard W; Heemskerk, Johan W M; Cosemans, Judith M E M (2014). Identification of platelet function defects by multi-parameter assessment of thrombus formation. Nature communications, 5, p. 4257. Nature Publishing Group 10.1038/ncomms5257
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2014_Nat Commun_Identification of platelet_Clemetson.pdf - Published Version Available under License Creative Commons: Attribution (CC-BY). Download (1MB) | Preview |
Assays measuring platelet aggregation (thrombus formation) at arterial shear rate mostly use collagen as only platelet-adhesive surface. Here we report a multi-surface and multi-parameter flow assay to characterize thrombus formation in whole blood from healthy subjects and patients with platelet function deficiencies. A systematic comparison is made of 52 adhesive surfaces with components activating the main platelet-adhesive receptors, and of eight output parameters reflecting distinct stages of thrombus formation. Three types of thrombus formation can be identified with a predicted hierarchy of the following receptors: glycoprotein (GP)VI, C-type lectin-like receptor-2 (CLEC-2)>GPIb>α6β1, αIIbβ3>α2β1>CD36, α5β1, αvβ3. Application with patient blood reveals distinct abnormalities in thrombus formation in patients with severe combined immune deficiency, Glanzmann's thrombasthenia, Hermansky-Pudlak syndrome, May-Hegglin anomaly or grey platelet syndrome. We suggest this test may be useful for the diagnosis of patients with suspected bleeding disorders or a pro-thrombotic tendency.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
04 Faculty of Medicine > Pre-clinic Human Medicine > Theodor Kocher Institute 04 Faculty of Medicine > Department of Haematology, Oncology, Infectious Diseases, Laboratory Medicine and Hospital Pharmacy (DOLS) > Clinic of Haematology and Central Haematological Laboratory |
UniBE Contributor: |
Clemetson, Kenneth John |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
2041-1723 |
Publisher: |
Nature Publishing Group |
Language: |
English |
Submitter: |
Verena Zwahlen |
Date Deposited: |
17 Mar 2015 14:38 |
Last Modified: |
05 Dec 2022 14:42 |
Publisher DOI: |
10.1038/ncomms5257 |
PubMed ID: |
25027852 |
Uncontrolled Keywords: |
thrombus formation |
BORIS DOI: |
10.7892/boris.64630 |
URI: |
https://boris.unibe.ch/id/eprint/64630 |
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