SNARE-fusion mediated insertion of membrane proteins into native and artificial membranes

Nordlund, Gustav; Brzezinski, Peter; von Ballmoos, Christoph (2014). SNARE-fusion mediated insertion of membrane proteins into native and artificial membranes. Nature communications, 5(1) Nature Publishing Group 10.1038/ncomms5303

Text ncomms5303.pdf - Published Version Restricted to registered users only Available under License Publisher holds Copyright. Download (421kB)

Membrane proteins carry out functions such as nutrient uptake, ATP synthesis or transmembrane signal transduction. An increasing number of reports indicate that cellular processes are underpinned by regulated interactions between these proteins. Consequently, functional studies of these networks at a molecular level require co-reconstitution of the interacting components. Here, we report a SNARE protein-based method for incorporation of multiple membrane proteins into artificial membrane vesicles of well-defined composition, and for delivery of large water-soluble substrates into these vesicles. The approach is used for in vitro reconstruction of a fully functional bacterial respiratory chain from purified components. Furthermore, the method is used for functional incorporation of the entire F1F0 ATP synthase complex into native bacterial membranes from which this component had been genetically removed. The novel methodology offers a tool to investigate complex interaction networks between membrane-bound proteins at a molecular level, which is expected to generate functional insights into key cellular functions.

Interest & Impact

Downloads

4 since deposited on 26 Mar 2015
0 in the past 12 months

Citations

5 Citations in Web of Science ®
6 Citations in Scopus

Search

in Google Scholar™

Services

Actions (login required)

Edit item

Actions (login required)

Edit item