Ignarski, Michael; Singh, Aditi; Swart, Estienne Carl; Arambasic, Miroslav; Sandoval, Pamela Yohanna de Lourdes; Nowacki, Mariusz (2014). Paramecium tetraurelia chromatin assembly factor-1-like protein PtCAF-1 is involved in RNA-mediated control of DNA elimination. Nucleic acids research, 42(19), pp. 11952-11964. Information Retrieval Ltd. 10.1093/nar/gku874
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Nucl. Acids Res.-2014-Ignarski-11952-64.pdf - Published Version Available under License Creative Commons: Attribution (CC-BY). Download (12MB) | Preview |
Genome-wide DNA remodelling in the ciliate Paramecium is ensured by RNA-mediated trans-nuclear crosstalk between the germline and the somatic genomes during sexual development. The rearrangements include elimination of transposable elements, minisatellites and tens of thousands non-coding elements called internally eliminated sequences (IESs). The trans-nuclear genome comparison process employs a distinct class of germline small RNAs (scnRNAs) that are compared against the parental somatic genome to select the germline-specific subset of scnRNAs that subsequently target DNA elimination in the progeny genome. Only a handful of proteins involved in this process have been identified so far and the mechanism of DNA targeting is unknown. Here we describe chromatin assembly factor-1-like protein (PtCAF-1), which we show is required for the survival of sexual progeny and localizes first in the parental and later in the newly developing macronucleus. Gene silencing shows that PtCAF-1 is required for the elimination of transposable elements and a subset of IESs. PTCAF-1 depletion also impairs the selection of germline-specific scnRNAs during development. We identify specific histone modifications appearing during Paramecium development which are strongly reduced in PTCAF-1 depleted cells. Our results demonstrate the importance of PtCAF-1 for the epigenetic trans-nuclear cross-talk mechanism.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
08 Faculty of Science > Department of Biology > Institute of Cell Biology > Ciliate 08 Faculty of Science > Department of Biology > Institute of Cell Biology |
UniBE Contributor: |
Ignarski, Michael, Singh, Aditi, Swart, Estienne Carl, Arambasic, Miroslav, Sandoval, Pamela Yohanna de Lourdes, Nowacki, Mariusz |
Subjects: |
500 Science > 570 Life sciences; biology |
ISSN: |
0305-1048 |
Publisher: |
Information Retrieval Ltd. |
Language: |
English |
Submitter: |
Mariusz Nowacki |
Date Deposited: |
11 Aug 2015 14:18 |
Last Modified: |
05 Dec 2022 14:48 |
Publisher DOI: |
10.1093/nar/gku874 |
PubMed ID: |
25270876 |
BORIS DOI: |
10.7892/boris.70923 |
URI: |
https://boris.unibe.ch/id/eprint/70923 |
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