Yusuf, Noor A; Green, Judith L; Wall, Richard J; Knuepfer, Ellen; Moon, Robert W; Schulte-Huxel, Christina; Limenitakis Stanway, Rebecca; Martin, Stephen R; Howell, Steven A; Douse, Christopher H; Cota, Ernesto; Tate, Edward W; Tewari, Rita; Holder, Anthony A (2015). The Plasmodium Class XIV Myosin, MyoB, Has a Distinct Subcellular Location in Invasive and Motile Stages of the Malaria Parasite and an Unusual Light Chain. Journal of biological chemistry, 290(19), pp. 12147-12164. American Society for Biochemistry and Molecular Biology 10.1074/jbc.M115.637694
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Myosin B (MyoB) is one of the two short class XIV myosins encoded in the Plasmodium genome. Class XIV myosins are characterized by a catalytic "head," a modified "neck," and the absence of a "tail" region. Myosin A (MyoA), the other class XIV myosin in Plasmodium, has been established as a component of the glideosome complex important in motility and cell invasion, but MyoB is not well characterized. We analyzed the properties of MyoB using three parasite species as follows: Plasmodium falciparum, Plasmodium berghei, and Plasmodium knowlesi. MyoB is expressed in all invasive stages (merozoites, ookinetes, and sporozoites) of the life cycle, and the protein is found in a discrete apical location in these polarized cells. In P. falciparum, MyoB is synthesized very late in schizogony/merogony, and its location in merozoites is distinct from, and anterior to, that of a range of known proteins present in the rhoptries, rhoptry neck or micronemes. Unlike MyoA, MyoB is not associated with glideosome complex proteins, including the MyoA light chain, myosin A tail domain-interacting protein (MTIP). A unique MyoB light chain (MLC-B) was identified that contains a calmodulin-like domain at the C terminus and an extended N-terminal region. MLC-B localizes to the same extreme apical pole in the cell as MyoB, and the two proteins form a complex. We propose that MLC-B is a MyoB-specific light chain, and for the short class XIV myosins that lack a tail region, the atypical myosin light chains may fulfill that role.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
08 Faculty of Science > Department of Biology > Institute of Cell Biology 08 Faculty of Science > Department of Biology > Institute of Cell Biology > Malaria |
UniBE Contributor: |
Limenitakis, Rebecca Rachel |
Subjects: |
500 Science > 570 Life sciences; biology 500 Science |
ISSN: |
0021-9258 |
Publisher: |
American Society for Biochemistry and Molecular Biology |
Language: |
English |
Submitter: |
Volker Heussler |
Date Deposited: |
10 Sep 2015 15:01 |
Last Modified: |
02 Mar 2023 23:26 |
Publisher DOI: |
10.1074/jbc.M115.637694 |
PubMed ID: |
25802338 |
Uncontrolled Keywords: |
Invasion, Malaria, Molecular Motor, Myosin, Parasite, Peptide Interaction, Plasmodium, Myosin Light Chain |
BORIS DOI: |
10.7892/boris.71393 |
URI: |
https://boris.unibe.ch/id/eprint/71393 |