Fasler-Kan, Elizaveta; Baiken, Yeldar; Vorobjev, Ivan A.; Barteneva, Natasha S. (2015). Analysis of Nucleocytoplasmic Protein Shuttling by Imaging Flow Cytometry. In: Barteneva, Natasha S.; Vorobjev, Ivan A. (eds.) Imaging Flow Cytometry - Methods and Protocols. Methods in Molecular Biology: Vol. 1389 (pp. 127-137). New York: Springer Science + Business Media (Humana Press) 10.1007/978-1-4939-3302-0_8
Full text not available from this repository. (Request a copy)Many intracellular signal transduction events involve the reversible shuttling of proteins between the cytoplasm and the nucleus. Study of these processes requires imaging information on the protein localization in a given cell and a large number of measurements to obtain sufficient statistics on the protein localization in the whole population. The protocol describes method for quantitative imaging flow cytometry analysis of intracellular distribution of NF-kappaB in ARPE-19 cells stained with specific fluorochrome-conjugated antibodies. The described technique alone or in combination with standard flow cytometry methods can be applied to study any protein undergoing translocation from cytoplasm into the nucleus in a variety of cell lines as well as in heterogeneous primary cell populations
Item Type: |
Book Section (Book Chapter) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > Department of Gynaecology, Paediatrics and Endocrinology (DFKE) > Clinic of Paediatric Surgery |
UniBE Contributor: |
Fasler-Kan, Elizaveta |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
1064-3745 |
ISBN: |
978-1-4939-3300-6 |
Series: |
Methods in Molecular Biology |
Publisher: |
Springer Science + Business Media (Humana Press) |
Language: |
English |
Submitter: |
Christoph Steffen |
Date Deposited: |
15 Feb 2016 09:50 |
Last Modified: |
05 Dec 2022 14:51 |
Publisher DOI: |
10.1007/978-1-4939-3302-0_8 |
Uncontrolled Keywords: |
Nucleocytoplasmic translocation; Nucleocytoplasmic shuttling; Imaging cytometry; Flow cytometry; NF-kappaB; ARPE-19 cell line; Retinal pigment cells |
URI: |
https://boris.unibe.ch/id/eprint/76188 |
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