Marcoli, Natalie; Østensen, Monika; Portmann-Lanz, C Bettina; Surbek, Daniel; Villiger, Peter; Förger, Frauke (2015). Differential influence of maternal and fetal pregnancy factors on the in-vitro induction of human regulatory T cells: a preliminary study. Swiss medical weekly, 145(w14172), w14172. EMH Schweizerischer Ärzteverlag 10.4414/smw.2015.14172
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PROBLEM
Given the important role of regulatory T cells (Treg) for successful pregnancy, the ability of soluble maternal and fetal pregnancy factors to induce human Treg was investigated.
METHOD OF STUDY
Peripheral blood mononuclear cells (PBMCs) or isolated CD4+CD25‒ cells were cultured in the presence of pooled second or third trimester pregnancy sera, steroid hormones or supernatants from placental explants, and the numbers and function of induced CD4+CD25+FOXP3+ Treg were analysed.
RESULTS
Third trimester pregnancy sera and supernatants of early placental explants, but not sex steroid hormones, induced an increase of Tregs from PBMCs. Early placental supernatant containing high levels of tumour necrosis factor-α, interferon-γ, interleukins -1, -6 and -17, soluble human leucocyte antigen-G, and transforming growth factor-β1, increased the proportion of Treg most effectively and was able to induce interleukin-10-secreting-Treg from CD4+CD25‒cells.
CONCLUSIONS
Compared with circulating maternal factors, placental- and fetal-derived factors appear to exert a more powerful effect on numerical changes of Treg, thereby supporting fetomaternal tolerance during human pregnancy.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
04 Faculty of Medicine > Department of Dermatology, Urology, Rheumatology, Nephrology, Osteoporosis (DURN) > Clinic of Rheumatology and Immunology 04 Faculty of Medicine > Department of Gynaecology, Paediatrics and Endocrinology (DFKE) > Clinic of Gynaecology |
UniBE Contributor: |
Surbek, Daniel, Villiger, Peter Matthias, Förger, Frauke |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
1424-7860 |
Publisher: |
EMH Schweizerischer Ärzteverlag |
Language: |
English |
Submitter: |
Monika Zehr |
Date Deposited: |
25 Feb 2016 10:59 |
Last Modified: |
05 Dec 2022 14:53 |
Publisher DOI: |
10.4414/smw.2015.14172 |
PubMed ID: |
26230352 |
BORIS DOI: |
10.7892/boris.77776 |
URI: |
https://boris.unibe.ch/id/eprint/77776 |
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