Occurrence and characteristics of the mitochondrial permeability transition in plants

Arpagaus, Silvio; Rawyler, André; Brändle, Roland (2002). Occurrence and characteristics of the mitochondrial permeability transition in plants. Journal of biological chemistry, 277(3), pp. 1780-1787. American Society for Biochemistry and Molecular Biology 10.1074/jbc.M109416200

Text JBiolChem_277_1780.pdf - Published Version Restricted to registered users only Available under License Publisher holds Copyright. Download (207kB) | Request a copy

dThe behavior of purified potato mitochondria toward the main effectors of the animal mitochondrial permeability transition has been studied by light scattering, fluorescence, SDS-polyacrylamide gel electrophoresis, and immunoblotting techniques. The addition of Ca2+ induces a phosphate-dependent swelling that is fully inhibited by cyclosporin A if dithioerythritol is present. Mg2+ cannot be substituted for Ca2+ but competes with it. Disruption of the outer membrane and release of several proteins, including cytochrome c, occur upon completion of swelling. Ca2+-induced swelling is delayed and its rate is decreased when pH is shifted from 7.4 to 6.6. It is accelerated by diamide, phenylarsine oxide, and linolenic acid. In the absence of Ca2+, however, linolenic acid (less than or equal to20 mum) rapidly dissipates the succinate-driven membrane potential while having no effect on mitochondrial volume. Anoxic conditions favor in vitro swelling and the concomitant release of cytochrome c and of other proteins in a pH-dependent way. These data indicate that the classical mitochondrial permeability transition occurs also in plants. This may have important implications for our understanding of cell stress and death processes.

Interest & Impact

Downloads

3 since deposited on 06 Jul 2017
0 in the past 12 months

Citations

5 Citations in Web of Science ®
6 Citations in Scopus

Search

in Google Scholar™

Services

Actions (login required)

Edit item

Actions (login required)

Edit item