Bochkov, Valery; Schoenenberger, Andreas; Oskolkova, Olga; Toth, Ursula; Stöckl, Johannes; Majdic, Otto; Daci, Armond; Resink, Thérèse J; Erne, Paul; Philippova, Maria (2016). Novel immune assay for quantification of plasma protective capacity against oxidized phospholipids. Biomarkers in medicine, 10(8), pp. 797-810. Future Medicine 10.2217/bmm-2016-0096
Full text not available from this repository.AIM
Oxidized phospholipids (OxPL) are the major pathogenic component of oxidized low-density lipoproteins (OxLDL). Endogenous anti-OxPL activity, defined as the ability to neutralize adverse effects of oxidized lipids, may have biomarker potential.
METHODS & RESULTS
Using two anti-OxPL monoclonal antibodies (commercial mAB-E06 and custom mAB-509) we developed a novel ELISA that measures the global capacity of plasma to inactivate OxPL. Preincubation of OxLDL with plasma inhibits its binding of anti-OxPL mABs. This phenomenon ('masking') reflects anti-OxPL plasma activity. A pilot clinical application of the assay revealed reduced anti-OxPL activity in hypertension, coronary artery disease, acute coronary syndrome and diabetes.
CONCLUSION
Inadequate anti-OxPL protection may contribute to cardiovascular disease and have biomarker potential in conditions associated with abnormal lipid peroxidation.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > Department of General Internal Medicine (DAIM) > Geriatric Clinic |
UniBE Contributor: |
Schoenenberger, Andreas |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
1752-0371 |
Publisher: |
Future Medicine |
Language: |
English |
Submitter: |
Rebecca Rufer |
Date Deposited: |
16 May 2017 07:14 |
Last Modified: |
05 Dec 2022 15:02 |
Publisher DOI: |
10.2217/bmm-2016-0096 |
PubMed ID: |
27416002 |
Uncontrolled Keywords: |
ELISA; anti-oxidized phospholipid antibodies; anti-oxidized phospholipid defense; biomarker potential; cardiovascular disease; oxidized phospholipids |
URI: |
https://boris.unibe.ch/id/eprint/95547 |
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