Profiling Transposable Elements and Their Epigenetic Effects in Non-model Species

Parisod, Christian Gérard (2016). Profiling Transposable Elements and Their Epigenetic Effects in Non-model Species. In: Kovalchuk, Igor (ed.) Plant Epigenetics. Methods in Molecular Biology: Vol. 1456 (pp. 243-250). Boston, MA: Springer US 10.1007/978-1-4899-7708-3_19

Text 243-250 aus Plant Epigenetics - Kovalchuk, Igor (2016).pdf - Published Version Restricted to registered users only Available under License Publisher holds Copyright. Download (230kB)

Taking transposable elements into consideration in surveys of genetic and epigenetic variation remains challenging in species lacking a high-quality reference genome. Here, molecular techniques reducing genome complexity and specifically targeting restructuring and methylation changes in TE genome fractions are described. In particular, methyl-sensitive transposon display (MSTD) uses isoschizomers and PCR amplifications to assess the methylation environment of TE insertions. MSTD offers reliable insights into genome-wide epigenetic changes associated with TEs, especially when used together with similar techniques tracking random sequences.

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