Profiling Transposable Elements and Their Epigenetic Effects in Non-model Species

Parisod, Christian Gérard (2016). Profiling Transposable Elements and Their Epigenetic Effects in Non-model Species. In: Kovalchuk, Igor (ed.) Plant Epigenetics. Methods in Molecular Biology: Vol. 1456 (pp. 243-250). Boston, MA: Springer US 10.1007/978-1-4899-7708-3_19

[img] Text
243-250 aus Plant Epigenetics - Kovalchuk, Igor (2016).pdf - Published Version
Restricted to registered users only
Available under License Publisher holds Copyright.

Download (230kB)

Taking transposable elements into consideration in surveys of genetic and epigenetic variation remains challenging in species lacking a high-quality reference genome. Here, molecular techniques reducing genome complexity and specifically targeting restructuring and methylation changes in TE genome fractions are described. In particular, methyl-sensitive transposon display (MSTD) uses isoschizomers and PCR amplifications to assess the methylation environment of TE insertions. MSTD offers reliable insights into genome-wide epigenetic changes associated with TEs, especially when used together with similar techniques tracking random sequences.

Item Type:

Book Section (Book Chapter)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS)

UniBE Contributor:

Parisod, Christian Gérard

Subjects:

500 Science > 580 Plants (Botany)

ISBN:

978-1-4899-7706-9

Series:

Methods in Molecular Biology

Publisher:

Springer US

Language:

English

Submitter:

Peter Alfred von Ballmoos-Haas

Date Deposited:

30 Jan 2018 08:08

Last Modified:

05 Dec 2022 15:07

Publisher DOI:

10.1007/978-1-4899-7708-3_19

PubMed ID:

27770371

Uncontrolled Keywords:

Transposons; Transposable elements; Epigenetic effects; Plants; Methyl-sensitive transposon display

BORIS DOI:

10.7892/boris.106382

URI:

https://boris.unibe.ch/id/eprint/106382

Actions (login required)

Edit item Edit item
Provide Feedback