Profiling Transposable Elements and Their Epigenetic Effects in Non-model Species

Parisod, Christian Gérard (2016). Profiling Transposable Elements and Their Epigenetic Effects in Non-model Species. In: Kovalchuk, Igor (ed.) Plant Epigenetics. Methods in Molecular Biology: Vol. 1456 (pp. 243-250). Boston, MA: Springer US 10.1007/978-1-4899-7708-3_19

Text 243-250 aus Plant Epigenetics - Kovalchuk, Igor (2016).pdf - Published Version Restricted to registered users only Available under License Publisher holds Copyright. Download (230kB) | Request a copy

Taking transposable elements into consideration in surveys of genetic and epigenetic variation remains challenging in species lacking a high-quality reference genome. Here, molecular techniques reducing genome complexity and specifically targeting restructuring and methylation changes in TE genome fractions are described. In particular, methyl-sensitive transposon display (MSTD) uses isoschizomers and PCR amplifications to assess the methylation environment of TE insertions. MSTD offers reliable insights into genome-wide epigenetic changes associated with TEs, especially when used together with similar techniques tracking random sequences.

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