Barbier, François; Ruppé, Etienne; Hernandez, David; Lebeaux, David; Francois, Patrice; Felix, Benjamin; Desprez, Adeline; Maiga, Aminata; Woerther, Paul-Louis; Gaillard, Kevin; Jeanrot, Cécile; Wolff, Michel; Schrenzel, Jacques; Andremont, Antoine; Ruimy, Raymond (2010). Methicillin-Resistant Coagulase-Negative Staphylococci in the Community: High Homology of SCCmec IVa between Staphylococcus epidermidis and Major Clones of Methicillin-Resistant Staphylococcus aureus. Journal of infectious diseases, 202(2), pp. 270-281. Oxford University Press 10.1086/653483
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Background. Data on community spread of methicillin-resistant coagulase-negative staphylococci (MR-CoNS) are scarce. We assessed their potential role as a reservoir of staphylococcal cassette chromosome mec (SCCmec) IVa, the leading SCCmec subtype in community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA).
Methods. Nasal carriage of MR-CoNS was prospectively investigated in 291 adults at hospital admission. MR-CoNS were characterized by SCCmec typing, long-range polymerase chain reaction (PCR) for SCCmec IV, and multiple-locus variable-number tandem repeat analysis (MLVA) for Staphylococcus epidermidis (MRSE) strains. Three SCCmec IVa elements were fully sequenced.
Results. The carriage rate of MR-CoNS was 19.2% (25.9% and 16.5% in patients with and patients without previous exposure to the health care system, respectively; P = .09). MR-CoNS strains (n=83, including 58 MRSE strains with highly heterogeneous MLVA patterns) carried SCCmec type IVa (n=9, all MRSE), other SCCmec IV subtypes (n=9, including 7 MRSE), other SCCmec types (n=15), and nontypeable SCCmec (n=50). Long-range PCR indicated structural homology between SCCmec IV in MRSE and that in MRSA. Complete sequences of SCCmec IVa from 3 MRSE strains were highly homologous to those available for CA-MRSA, including major clones USA300 and USA400.
Conclusions. MR-CoNS are probably disseminated in the community, notably in subjects without previous exposure to the health care system. MRSE, the most prevalent species, may act as a reservoir of SCCmec IVa for CA-MRSA.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases > Research 04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases |
UniBE Contributor: |
Schrenzel, Jacques |
ISSN: |
0022-1899 |
Publisher: |
Oxford University Press |
Language: |
English |
Submitter: |
Marceline Brodmann |
Date Deposited: |
09 Nov 2021 17:38 |
Last Modified: |
05 Dec 2022 15:13 |
Publisher DOI: |
10.1086/653483 |
BORIS DOI: |
10.7892/boris.115596 |
URI: |
https://boris.unibe.ch/id/eprint/115596 |
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