Spider Neurotoxins, Short Linear Cationic Peptides and Venom Protein Classification Improved by an Automated Competition between Exhaustive Profile HMM Classifiers

Koua, Dominique Kadio; Kuhn-Nentwig, Lucia Gerda (2017). Spider Neurotoxins, Short Linear Cationic Peptides and Venom Protein Classification Improved by an Automated Competition between Exhaustive Profile HMM Classifiers. Toxins, 9(8), p. 245. MDPI 10.3390/toxins9080245

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Spider venoms are rich cocktails of bioactive peptides, proteins, and enzymes that are being intensively investigated over the years. In order to provide a better comprehension of that richness, we propose a three-level family classification system for spider venom components. This classification is supported by an exhaustive set of 219 new profile hidden Markov models (HMMs) able to attribute a given peptide to its precise peptide type, family, and group. The proposed classification has the advantages of being totally independent from variable spider taxonomic names and can easily evolve. In addition to the new classifiers, we introduce and demonstrate the efficiency of hmmcompete, a new standalone tool that monitors HMM-based family classification and, after post-processing the result, reports the best classifier when multiple models produce significant scores towards given peptide queries. The combined used of hmmcompete and the new spider venom component-specific classifiers demonstrated 96% sensitivity to properly classify all known spider toxins from the UniProtKB database. These tools are timely regarding the important classification needs caused by the increasing number of peptides and proteins generated by transcriptomic projects.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Ecology and Evolution (IEE)

UniBE Contributor:

Koua, Dominique Kadio and Kuhn-Nentwig, Lucia Gerda

Subjects:

500 Science > 570 Life sciences; biology

ISSN:

2072-6651

Publisher:

MDPI

Language:

English

Submitter:

Alexander Strauss

Date Deposited:

08 Jun 2018 14:15

Last Modified:

25 Oct 2019 06:40

Publisher DOI:

10.3390/toxins9080245

BORIS DOI:

10.7892/boris.116204

URI:

https://boris.unibe.ch/id/eprint/116204

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