Exploring the virome of cattle with non-suppurative encephalitis of unknown etiology by metagenomics

Wüthrich, Daniel; Boujon, Céline; Truchet, Laura Rosyne Hedwig; Selimovic-Hamza, Senija; Oevermann, Anna; Bouzalas, Ilias; Bruggmann, Rémy; Seuberlich, Torsten (2016). Exploring the virome of cattle with non-suppurative encephalitis of unknown etiology by metagenomics. Virology, 493, pp. 22-30. Academic Press 10.1016/j.virol.2016.03.009

[img]
Preview
Text
1-s2.0-S0042682216300290-main.pdf - Published Version
Available under License Creative Commons: Attribution-Noncommercial-No Derivative Works (CC-BY-NC-ND).

Download (1MB) | Preview

Non-suppurative encephalitis is one of the most frequent pathological diagnosis in cattle with neurological disease, but there is a gap in the knowledge on disease-associated pathogens. In order to identify viruses that are associated with non-suppurative encephalitis in cattle, we used a viral metagenomics approach on a sample set of 16 neurologically-diseased cows. We detected six virus candidates: parainfluenza virus 5 (PIV-5), bovine astrovirus CH13/NeuroS1 (BoAstV-CH13/NeuroS1), bovine polyomavirus 2 (BPyV-2 SF), ovine herpesvirus 2 (OvHV-2), bovine herpesvirus 6 (BHV-6) and a novel bovine betaretrovirus termed BoRV-CH15. In a case-control study using PCR, BoAstV-CH13 (p=0.046), BoPV-2 SF (p=0.005) and BoHV-6 (p=4.3E-05) were statistically associated with the disease. These data expand our knowledge on encephalitis-associated pathogens in cattle and point to the value of NGS in resolving complex infection scenarios in a clinical disease setting.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > NeuroCenter
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Experimental Clinical Research

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Wüthrich, Daniel; Boujon, Céline; Truchet, Laura Rosyne Hedwig; Selimovic-Hamza, Senija; Oevermann, Anna; Bouzalas, Ilias; Bruggmann, Rémy and Seuberlich, Torsten

Subjects:

500 Science > 570 Life sciences; biology

ISSN:

0042-6822

Publisher:

Academic Press

Language:

English

Submitter:

Torsten Seuberlich

Date Deposited:

07 Jun 2018 09:35

Last Modified:

07 Jun 2018 09:40

Publisher DOI:

10.1016/j.virol.2016.03.009

PubMed ID:

26994586

BORIS DOI:

10.7892/boris.117027

URI:

https://boris.unibe.ch/id/eprint/117027

Actions (login required)

Edit item Edit item
Provide Feedback