mRNA-specific translation regulation by a ribosome-associated ncRNA in Haloferax volcanii

Wyss, Leander Nicolas; Waser, Melanie; Gebetsberger, Jennifer; Zywicki, Marek; Polacek, Norbert (2018). mRNA-specific translation regulation by a ribosome-associated ncRNA in Haloferax volcanii. Scientific Reports, 8(1) Nature Publishing Group 10.1038/s41598-018-30332-w

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Regulation of gene expression at the translational level allows rapid adaptation of cellular proteomes to quickly changing environmental conditions and is thus central for prokaryotic organisms. Small non-coding RNAs (sRNAs) have been reported to effectively orchestrate translation control in bacteria and archaea mainly by targeting mRNAs by partial base complementarity. Here we report an unprecedented mechanism how sRNAs are capable of modulating protein biosynthesis in the halophilic archaeon Haloferax volcanii. By analyzing the ribosome-associated ncRNAs (rancRNAs) under different stress conditions we identified an intergenic sRNA, termed rancRNA_s194, that is primarily expressed during exponential growth under all tested conditions. By interaction with the ribosome rancRNA_s194 inhibits peptide bond formation and protein synthesis in vitro but appears to target a specific mRNA in vivo. The respective knock-out strain shows a reduced lag phase in media containing xylose as sole carbon source and outcompetes the wildtype cells under these conditions. Mass spectrometry, polysome profiling and mRNA binding competition experiments suggest that rancRNA_s194 prevents the cstA mRNA from being efficiently translated by H. volcanii ribosomes. These findings enlarge the regulatory repertoire of archaeal sRNAs in modulating post-transcriptional gene expression.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Departement of Chemistry and Biochemistry

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Wyss, Leander Nicolas and Polacek, Norbert

Subjects:

500 Science > 570 Life sciences; biology
500 Science > 540 Chemistry

ISSN:

2045-2322

Publisher:

Nature Publishing Group

Language:

English

Submitter:

Christina Schüpbach

Date Deposited:

03 Sep 2018 11:20

Last Modified:

23 Oct 2019 19:34

Publisher DOI:

10.1038/s41598-018-30332-w

BORIS DOI:

10.7892/boris.119693

URI:

https://boris.unibe.ch/id/eprint/119693

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