Precise Delineation and Transcriptional Characterization of Bovine Blood Dendritic-Cell and Monocyte Subsets.

Talker, Stephanie; Baumann, Arnaud; Barut, Güliz Tuba; Keller, Irene; Bruggmann, Rémy; Summerfield, Artur (2018). Precise Delineation and Transcriptional Characterization of Bovine Blood Dendritic-Cell and Monocyte Subsets. Frontiers in immunology, 9(2505), p. 2505. Frontiers Research Foundation 10.3389/fimmu.2018.02505

[img]
Preview
Text
fimmu-09-02505.pdf - Published Version
Available under License Creative Commons: Attribution (CC-BY).

Download (4MB) | Preview

A clear-cut delineation of bovine dendritic cells (DC) from monocytes has proved challenging, given the high phenotypic and functional plasticity of these innate immune cells and the marked phenotypic differences between species. Here, we demonstrate that, based on expression of Flt3, CD172a, CD13, and CD4, a precise identification of bovine blood conventional DC type 1 and 2 (cDC1, cDC2), plasmacytoid DC (pDC), and monocytes is possible with cDC1 being Flt3CD172aCD13CD4, cDC2 being Flt3CD172aCD13CD4, pDC being Flt3CD172aCD13CD4, and monocytes being Flt3CD172aCD13CD4. The phenotype of these subsets was characterized in further detail, and a subset-specific differential expression of CD2, CD5, CD11b, CD11c, CD14, CD16, CD26, CD62L, CD71, CD163, and CD205 was found. Subset identity was confirmed by transcriptomic analysis and subset-specific transcription of conserved key genes. We also sorted monocyte subsets based on their differential expression of CD14 and CD16. Classical monocytes (CD14CD16) clustered clearly apart from the two CD16 monocyte subsets probably representing intermediate and non-classical monocytes described in human. The transcriptomic data also revealed differential gene transcription for molecules involved in antigen presentation, pathogen sensing, and migration, and therefore gives insights into functional differences between bovine DC and monocyte subsets. The identification of cell-type- and subset-specific gene transcription will assist in the quest for "marker molecules" that-when targeted by flow cytometry-will greatly facilitate research on bovine DC and monocytes. Overall, species comparisons will elucidate basic principles of DC and monocyte biology and will help to translate experimental findings from one species to another.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR)
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Virology and Immunology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Talker, Stephanie; Baumann, Arnaud; Barut, Güliz Tuba; Keller, Irene; Bruggmann, Rémy and Summerfield, Artur

Subjects:

600 Technology > 610 Medicine & health
600 Technology > 630 Agriculture
500 Science > 570 Life sciences; biology

ISSN:

1664-3224

Publisher:

Frontiers Research Foundation

Language:

English

Submitter:

Achim Braun Parham

Date Deposited:

12 Apr 2019 13:38

Last Modified:

14 Apr 2019 02:35

Publisher DOI:

10.3389/fimmu.2018.02505

PubMed ID:

30425716

Uncontrolled Keywords:

cDC1 cDC2 cattle dendritic cells monocytes pDC transcriptome

BORIS DOI:

10.7892/boris.127522

URI:

https://boris.unibe.ch/id/eprint/127522

Actions (login required)

Edit item Edit item
Provide Feedback