Effects of gold and PCL- or PLLA-coated silica nanoparticles on brain endothelial cells and the blood–brain barrier

Bittner, Aniela; Ducray, Angélique; Widmer, Hans Rudolf; Stoffel, Michael H; Mevissen, Meike (2019). Effects of gold and PCL- or PLLA-coated silica nanoparticles on brain endothelial cells and the blood–brain barrier. Beilstein journal of nanotechnology, 10, pp. 941-954. Beilstein-Institut zur Förderung der Chemischen Wissenschaften 10.3762/bjnano.10.95

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Nanomedicine is a constantly expanding field, facilitating and improving diagnosis and treatment of diseases. As nanomaterials are foreign objects, careful evaluation of their toxicological and functional aspects prior to medical application is imperative. In this study, we aimed to determine the effects of gold and polymer-coated silica nanoparticles used in laser tissue soldering on brain endothelial cells and the blood–brain barrier using rat brain capillary endothelial cells (rBCEC4). All types of nanoparticles were taken up time-dependently by the rBCEC4 cells, albeit to a different extent, causing a time- and concentration-dependent decrease in cell viability. Nanoparticle exposure did not change cell proliferation, differentiation, nor did it induce inflammation. rBCEC4 cells showed blood–brain barrier characteristics including tight junctions. None of the nanoparticles altered the expression of tight junctions or impaired the blood–brain barrier permeability. The findings suggest that effects of these nanoparticles on the metabolic state of cells have to be further characterized before use for medical purposes.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Head Organs and Neurology (DKNS) > Clinic of Neurosurgery
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Veterinary Pharmacology and Toxicology
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Veterinary Anatomy
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH)
09 Interdisciplinary Units > Microscopy Imaging Center MIC

UniBE Contributor:

Bittner, Aniela; Ducray, Angélique; Widmer, Hans Rudolf; Stoffel, Michael Hubert and Mevissen, Meike

Subjects:

600 Technology > 630 Agriculture
500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

2190-4286

Publisher:

Beilstein-Institut zur Förderung der Chemischen Wissenschaften

Language:

English

Submitter:

Vanessa Alice Blum

Date Deposited:

05 Jun 2019 11:40

Last Modified:

07 Aug 2019 15:16

Publisher DOI:

10.3762/bjnano.10.95

PubMed ID:

31165021

Uncontrolled Keywords:

blood–brain barrier; laser tissue soldering; nanomedicine; nanoparticle uptake; rBCEC4 cells

BORIS DOI:

10.7892/boris.130478

URI:

https://boris.unibe.ch/id/eprint/130478

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