The Cre/loxP system in Giardia lamblia: genetic manipulations in a binucleate tetraploid protozoan

Wampfler, Petra B.; Faso, Carmen; Hehl, Adrian B. (2014). The Cre/loxP system in Giardia lamblia: genetic manipulations in a binucleate tetraploid protozoan. International journal for parasitology, 44(8), pp. 497-506. Elsevier 10.1016/j.ijpara.2014.03.008

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The bacteriophage-derived Cre/loxP system is a valuable tool that has revolutionised genetic and cell biological research in many organisms. We implemented this system in the intestinal parasite Giardia lamblia, an evolutionarily diverged protozoan whose binucleate and tetraploid genome organisation severely limits the application of reverse genetic approaches. We show that Cre-recombinase is functionally expressed in G. lamblia and demonstrate “recycling” of selectable markers. Providing the means for more complex and versatile genetic modifications, this technique massively increases the scope of functional investigations in G. lamblia and other protozoa with similar limitations with respect to genetic manipulation.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Cell Biology
08 Faculty of Science > Department of Biology > Institute of Cell Biology > Parasitologie

UniBE Contributor:

Faso, Carmen

Subjects:

500 Science > 570 Life sciences; biology
500 Science > 590 Animals (Zoology)

ISSN:

0020-7519

Publisher:

Elsevier

Language:

English

Submitter:

Carmen Faso

Date Deposited:

19 Sep 2019 14:16

Last Modified:

27 Oct 2019 19:18

Publisher DOI:

10.1016/j.ijpara.2014.03.008

PubMed ID:

24747534

BORIS DOI:

10.7892/boris.131609

URI:

https://boris.unibe.ch/id/eprint/131609

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