Wampfler, Petra B.; Faso, Carmen; Hehl, Adrian B. (2014). The Cre/loxP system in Giardia lamblia: genetic manipulations in a binucleate tetraploid protozoan. International journal for parasitology, 44(8), pp. 497-506. Elsevier 10.1016/j.ijpara.2014.03.008
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The bacteriophage-derived Cre/loxP system is a valuable tool that has revolutionised genetic and cell biological research in many organisms. We implemented this system in the intestinal parasite Giardia lamblia, an evolutionarily diverged protozoan whose binucleate and tetraploid genome organisation severely limits the application of reverse genetic approaches. We show that Cre-recombinase is functionally expressed in G. lamblia and demonstrate “recycling” of selectable markers. Providing the means for more complex and versatile genetic modifications, this technique massively increases the scope of functional investigations in G. lamblia and other protozoa with similar limitations with respect to genetic manipulation.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
08 Faculty of Science > Department of Biology > Institute of Cell Biology 08 Faculty of Science > Department of Biology > Institute of Cell Biology > Parasitologie |
UniBE Contributor: |
Faso, Carmen |
Subjects: |
500 Science > 570 Life sciences; biology 500 Science > 590 Animals (Zoology) |
ISSN: |
0020-7519 |
Publisher: |
Elsevier |
Language: |
English |
Submitter: |
Carmen Faso |
Date Deposited: |
19 Sep 2019 14:16 |
Last Modified: |
05 Dec 2022 15:29 |
Publisher DOI: |
10.1016/j.ijpara.2014.03.008 |
PubMed ID: |
24747534 |
BORIS DOI: |
10.7892/boris.131609 |
URI: |
https://boris.unibe.ch/id/eprint/131609 |
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