Peptide occurring in Enterobacteriaceae triggers Streptococcus pneumoniae cell death

Nasher, Fauzy; Kwun, Min Jung; Croucher, Nicholas J; Heller, Manfred; Hathaway, Lucy J. (2019). Peptide occurring in Enterobacteriaceae triggers Streptococcus pneumoniae cell death. Frontiers in cellular and infection microbiology, 9(320), p. 320. Frontiers 10.3389/fcimb.2019.00320

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Nonencapsulated Streptococcus pneumoniae often possess two genes, aliB-like ORF 1 and aliB-like ORF 2, in place of capsule genes. AliB-like ORF 1 is thought to encode a substrate binding protein of an ABC transporter which binds peptide SETTFGRDFN, found in 50S ribosomal subunit protein L4 of Enterobacteriaceae. Here, we investigated the effect of binding of AliB-like ORF 1 peptide on the transcriptome and proteome of nonencapsulated pneumococci. We found upregulation of gene expression of a metacaspase and a gene encoding N-acetylmuramoyl-L-alanine amidase, both of which are proposed to be involved in programmed cell death in prokaryotic cells. Proteome profiling indicated upregulation of transcriptional regulators and downregulation of metabolism-associated genes. Exposure to the peptide specifically triggered death in pneumococci which express AliB-like ORF 1, with the bacteria having an apoptotic appearance by electron microscopy. We propose that binding of the AliB-like ORF 1 peptide ligand by the pneumococcus signals a challenging environment with hostile bacterial species leading to death of a proportion of the pneumococcal population.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR)

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Nasher, Fauzy Negiub Ali; Heller, Manfred and Hathaway, Lucy Jane

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

2235-2988

Publisher:

Frontiers

Funders:

[UNSPECIFIED] Swiss National Science Foundation
[UNSPECIFIED] BBSRC

Language:

English

Submitter:

Lucy Jane Hathaway

Date Deposited:

18 Sep 2019 17:00

Last Modified:

12 Jan 2020 02:39

Publisher DOI:

10.3389/fcimb.2019.00320

PubMed ID:

31552200

BORIS DOI:

10.7892/boris.133297

URI:

https://boris.unibe.ch/id/eprint/133297

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