Bonetti, Sara; Hirschi, Stephan; Bosshart, Patrick D. (2020). Expression, purification and crystallization of an SLC16 monocarboxylate transporter family homologue specific for l-lactate. Protein expression and purification, 165, p. 105484. Elsevier 10.1016/j.pep.2019.105484
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l-lactate plays an important role as metabolite and signaling molecule in eukaryotes and bacteria. Monocarboxylate transporters (MCTs) of the SLC16 solute carrier family are responsible for the transport of l-lactate across eukaryotic and bacterial cell membranes. Here we report an efficient protocol for the expression and purification of an SLC16 family homologue in milligram amounts. The purified protein is stable and can thus be used for biochemical and structural studies as shown by successful crystallization.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Biochemistry and Molecular Medicine |
Graduate School: |
Graduate School for Cellular and Biomedical Sciences (GCB) |
UniBE Contributor: |
Bonetti, Sara, Hirschi, Stephan, Bosshart, Patrick |
Subjects: |
500 Science > 570 Life sciences; biology 600 Technology > 610 Medicine & health |
ISSN: |
1096-0279 |
Publisher: |
Elsevier |
Language: |
English |
Submitter: |
Barbara Franziska Järmann-Bangerter |
Date Deposited: |
27 Sep 2019 12:33 |
Last Modified: |
05 Dec 2022 15:30 |
Publisher DOI: |
10.1016/j.pep.2019.105484 |
PubMed ID: |
31513847 |
Uncontrolled Keywords: |
Expression of membrane proteins Membrane protein Monocarboxylate transporter Purification of membrane proteins SLC16 family Structure l-lactate transporter |
BORIS DOI: |
10.7892/boris.133500 |
URI: |
https://boris.unibe.ch/id/eprint/133500 |
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