Expression, purification and crystallization of an SLC16 monocarboxylate transporter family homologue specific for l-lactate.

Bonetti, Sara; Hirschi, Stephan; Bosshart, Patrick D. (2020). Expression, purification and crystallization of an SLC16 monocarboxylate transporter family homologue specific for l-lactate. Protein expression and purification, 165, p. 105484. Elsevier 10.1016/j.pep.2019.105484

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l-lactate plays an important role as metabolite and signaling molecule in eukaryotes and bacteria. Monocarboxylate transporters (MCTs) of the SLC16 solute carrier family are responsible for the transport of l-lactate across eukaryotic and bacterial cell membranes. Here we report an efficient protocol for the expression and purification of an SLC16 family homologue in milligram amounts. The purified protein is stable and can thus be used for biochemical and structural studies as shown by successful crystallization.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Biochemistry and Molecular Medicine

UniBE Contributor:

Bonetti, Sara; Hirschi, Stephan and Bosshart, Patrick

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

1096-0279

Publisher:

Elsevier

Language:

English

Submitter:

Barbara Järmann-Bangerter

Date Deposited:

27 Sep 2019 12:33

Last Modified:

24 Oct 2019 08:03

Publisher DOI:

10.1016/j.pep.2019.105484

PubMed ID:

31513847

Uncontrolled Keywords:

Expression of membrane proteins Membrane protein Monocarboxylate transporter Purification of membrane proteins SLC16 family Structure l-lactate transporter

BORIS DOI:

10.7892/boris.133500

URI:

https://boris.unibe.ch/id/eprint/133500

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