Expression, purification and crystallization of an SLC16 monocarboxylate transporter family homologue specific for l-lactate.

Bonetti, Sara; Hirschi, Stephan; Bosshart, Patrick D. (2020). Expression, purification and crystallization of an SLC16 monocarboxylate transporter family homologue specific for l-lactate. Protein expression and purification, 165, p. 105484. Elsevier 10.1016/j.pep.2019.105484

	Text Protein Expression.pdf - Published Version Restricted to registered users only Available under License Publisher holds Copyright. Download (952kB) | Request a copy
Preview	Text Bonetti_ProteinPurif_submitted.pdf - Accepted Version Available under License BORIS Standard License. Download (3MB) | Preview

l-lactate plays an important role as metabolite and signaling molecule in eukaryotes and bacteria. Monocarboxylate transporters (MCTs) of the SLC16 solute carrier family are responsible for the transport of l-lactate across eukaryotic and bacterial cell membranes. Here we report an efficient protocol for the expression and purification of an SLC16 family homologue in milligram amounts. The purified protein is stable and can thus be used for biochemical and structural studies as shown by successful crystallization.

Interest & Impact

Downloads

29 since deposited on 27 Sep 2019
16 in the past 12 months

Citations

5 Citations in Web of Science ®
6 Citations in Scopus

Search

in Google Scholar™

Services

Actions (login required)

Edit item

Actions (login required)

Edit item