Bonetti, Sara; Hirschi, Stephan; Bosshart, Patrick D. (2020). Expression, purification and crystallization of an SLC16 monocarboxylate transporter family homologue specific for l-lactate. Protein expression and purification, 165, p. 105484. Elsevier 10.1016/j.pep.2019.105484
![[img]](https://boris.unibe.ch/style/images/fileicons/text.png) |
Text
Protein Expression.pdf - Published Version Restricted to registered users only Available under License Publisher holds Copyright. Download (952kB) | Request a copy |
l-lactate plays an important role as metabolite and signaling molecule in eukaryotes and bacteria. Monocarboxylate transporters (MCTs) of the SLC16 solute carrier family are responsible for the transport of l-lactate across eukaryotic and bacterial cell membranes. Here we report an efficient protocol for the expression and purification of an SLC16 family homologue in milligram amounts. The purified protein is stable and can thus be used for biochemical and structural studies as shown by successful crystallization.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Biochemistry and Molecular Medicine |
UniBE Contributor: |
Bonetti, Sara; Hirschi, Stephan and Bosshart, Patrick |
Subjects: |
500 Science > 570 Life sciences; biology 600 Technology > 610 Medicine & health |
ISSN: |
1096-0279 |
Publisher: |
Elsevier |
Language: |
English |
Submitter: |
Barbara Järmann-Bangerter |
Date Deposited: |
27 Sep 2019 12:33 |
Last Modified: |
24 Oct 2019 08:03 |
Publisher DOI: |
10.1016/j.pep.2019.105484 |
PubMed ID: |
31513847 |
Uncontrolled Keywords: |
Expression of membrane proteins Membrane protein Monocarboxylate transporter Purification of membrane proteins SLC16 family Structure l-lactate transporter |
BORIS DOI: |
10.7892/boris.133500 |
URI: |
https://boris.unibe.ch/id/eprint/133500 |
Actions (login required)
 |
Edit item |